Linked Life Data

21330459

Source:http://linkedlifedata.com/resource/pubmed/id/21330459

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2011-4-8
pubmed:abstractText
Tissue transglutaminase (TG2) is a transpeptidase involved in protein cross-linking through generation of ?-(?-glutamyl)lysine isopeptide bonds. It also promotes cell adhesion through interaction with fibronectin and facilitates formation of fibronectin-integrin complexes. This interaction is involved in tumor cell adhesion to the matrix and in the process of tumor dissemination. Its inhibition by small molecules may therefore be useful in blocking metastasis. To that end, we screened more than 800,000 compounds following an in silico docking approach targeting two distinct cavities in the vicinity of the fibronectin-binding site on TG2. A total of 120 compounds were acquired and tested in cell culture-based assays for inhibition of ovarian tumor cell adhesion and proliferation. Seven compounds showed more than 50% inhibition of cell adhesion at a concentration of 25 ?mol/L. A follow-up fluorescence polarization study revealed that one compound in particular (ITP-79) inhibited binding of a TG2 peptide to a 42-kDa fragment of fibronectin in a dose-dependent manner. This inhibition was confirmed in cancer cells by coimmunoprecipitation. A competition assay with surface plasmon resonance showed that ITP-79 modulated binding of TG2 to fibronectin. Direct binding of compounds that inhibited adhesion to TG2 were examined with differential scanning fluorimetry, which measures the effect of the compound on the melting temperature of the target. Two compounds, including ITP-79, reduced TG2 stabilization, mimicking the effects of GTP, a known negative allosteric regulator of TG2 enzymatic function. This suggests a potential allosteric mechanism for the compound in light of its distal target site.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1538-8514
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
10
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
626-36
pubmed:dateRevised
2011-11-7
pubmed:meshHeading
pubmed-meshheading:21330459-Amino Acid Sequence, pubmed-meshheading:21330459-Benzimidazoles, pubmed-meshheading:21330459-Binding Sites, pubmed-meshheading:21330459-Cell Adhesion, pubmed-meshheading:21330459-Cell Line, Tumor, pubmed-meshheading:21330459-Crystallization, pubmed-meshheading:21330459-Enzyme Inhibitors, pubmed-meshheading:21330459-Female, pubmed-meshheading:21330459-Fibronectins, pubmed-meshheading:21330459-Fluorescence Polarization, pubmed-meshheading:21330459-Humans, pubmed-meshheading:21330459-Immunoprecipitation, pubmed-meshheading:21330459-Models, Molecular, pubmed-meshheading:21330459-Molecular Sequence Data, pubmed-meshheading:21330459-Molecular Structure, pubmed-meshheading:21330459-Ovarian Neoplasms, pubmed-meshheading:21330459-Peptide Fragments, pubmed-meshheading:21330459-Protein Binding, pubmed-meshheading:21330459-Protein Structure, Secondary, pubmed-meshheading:21330459-Protein Structure, Tertiary, pubmed-meshheading:21330459-Surface Plasmon Resonance, pubmed-meshheading:21330459-Thiazolidines, pubmed-meshheading:21330459-Transglutaminases
pubmed:year
2011
pubmed:articleTitle
Targeting ovarian tumor cell adhesion mediated by tissue transglutaminase.
pubmed:affiliation
Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, 535 Barnhill Drive, RT473, Indianapolis, IN 46202, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't