Source:http://linkedlifedata.com/resource/pubmed/id/21324528
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
8
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| pubmed:dateCreated |
2011-3-14
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| pubmed:databankReference | |
| pubmed:abstractText |
This paper reports the cloning and sequencing of interleukin (IL)-23 p19 subunit for the first time within a non-mammalian species, the zebrafish (Danio rerio), which was discovered using a synteny approach. In addition, amino acid sequences were for IL-23 p19 subunits were also predicted from the stickleback, Fugu and Tetraodon genomes and included in this investigation. The zebrafish IL-23 p19 cDNA consisted of a 66bp 5' UTR, a 249bp 3' UTR and a single open reading frame of 567bp giving a predicted 188 aa IL-23 p19 molecule. Multiple alignment of zebrafish IL-23 p19, with other known IL-23 p19 and IL-12 p35 amino acid sequences revealed areas of amino acid conservation, such as the presence of four predicted ?-helixes, cysteines important for disulphide bond formation and the conservation of a tryptophan known to interact with the receptor. Amino acid homologies and phylogenetic analysis confirmed the relationship of the fish IL-23 p19 subunits with their mammalian homologues. All the teleost fish IL-23 p19 subunits were found to have 4 exons and 3 introns similar to that of human and mouse IL-23 p19 and a limited degree of synteny was found between the organisms for the regions containing the IL-23 p19 genes with only PAB-dependent poly(A)-specific ribonuclease subunit 2 (PAN2) and IL-23 p19 found in the same order on human chromosome 12 and all the fish genomes looked at. Lastly using real-time PCR, constitutive expression of IL-12 p40 and IL-23 p19 was observed in the kidney, liver, gut and muscle with IL-12 p40 expression higher than IL-23 p19. As soon as an hour after stimulation with LPS, there was an increase of IL-23 p19 in zebrafish leukocytes and an increase of IL-1?, IL-12 p40 and IL-23 p19 expression was found after infection of zebrafish for 1 or 6 days with Mycobaterium marinum strain E11.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Apr
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| pubmed:issn |
1872-9142
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| pubmed:author | |
| pubmed:copyrightInfo |
Copyright © 2011 Elsevier Ltd. All rights reserved.
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| pubmed:issnType |
Electronic
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| pubmed:volume |
48
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
981-91
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| pubmed:meshHeading |
pubmed-meshheading:21324528-Amino Acid Sequence,
pubmed-meshheading:21324528-Animals,
pubmed-meshheading:21324528-Base Sequence,
pubmed-meshheading:21324528-Chromosome Mapping,
pubmed-meshheading:21324528-Chromosomes, Human, Pair 12,
pubmed-meshheading:21324528-Cloning, Molecular,
pubmed-meshheading:21324528-Gene Expression Profiling,
pubmed-meshheading:21324528-Host-Pathogen Interactions,
pubmed-meshheading:21324528-Humans,
pubmed-meshheading:21324528-Inflammation Mediators,
pubmed-meshheading:21324528-Interleukin-23 Subunit p19,
pubmed-meshheading:21324528-Leukocytes,
pubmed-meshheading:21324528-Lipopolysaccharides,
pubmed-meshheading:21324528-Molecular Sequence Data,
pubmed-meshheading:21324528-Mycobacterium marinum,
pubmed-meshheading:21324528-Phylogeny,
pubmed-meshheading:21324528-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:21324528-Sequence Analysis, DNA,
pubmed-meshheading:21324528-Sequence Homology, Amino Acid,
pubmed-meshheading:21324528-Synteny,
pubmed-meshheading:21324528-Th17 Cells,
pubmed-meshheading:21324528-Zebrafish,
pubmed-meshheading:21324528-Zebrafish Proteins
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| pubmed:year |
2011
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| pubmed:articleTitle |
Discovery of zebrafish (Danio rerio) interleukin-23 alpha (IL-23?) chain, a subunit important for the formation of IL-23, a cytokine involved in the development of Th17 cells and inflammation.
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| pubmed:affiliation |
Scottish Fish Immunology Research Centre, School of Biological Sciences, Zoology Building, University of Aberdeen, Aberdeen, AB24 2TZ, UK.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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