Linked Life Data

21321486

Source:http://linkedlifedata.com/resource/pubmed/id/21321486

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2011-4-4
pubmed:abstractText
Recombinant adenovirus vectors (Ad) have been recognized as effective in vivo gene delivery vehicles and utilized as gene therapy agents for a number of cancers. The elucidation of viral entry mechanisms has allowed the development of recombinant vectors that exploit existing cell surface receptors to achieve entry into the cell. B lymphocytes are normally resistant to infection by adenovirus 5, likely due to the lack of the Coxsackie and Adenovirus receptor (CAR). Using reverse-transcriptase PCR and flow cytometry, the CD40 receptor has been shown to be expressed on many lymphoma cells. We exploited this finding to develop a gene therapy strategy for treatment of canine B cell lymphoma. Ad5 was targeted to cells expressing CD40 via CD40 ligand (CD40L) and was effective in infecting CD40-expressing control cells; however, both primary canine lymphoma cells and cell lines demonstrated limited evidence of transduction. Following receptor binding, adenovirus entry into cells may require interaction with ?(v)?(3/5) integrins; we demonstrate that canine lymphoma cells are deficient in these integrins. Reduced ?(v)?(3) integrin expression may render these cells incapable of internalizing Ad vectors. Thus, any viral targeting approaches for treatment of canine lymphoma must also take into account the potential lack of internalization signals.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1555-8576
pubmed:author
pubmed:issnType
Electronic
pubmed:day
1
pubmed:volume
11
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
651-8
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
Resistance of canine lymphoma cells to adenoviral infection due to reduced cell surface RGD binding integrins.
pubmed:affiliation
Scott Ritchey Research Center, College of Veterinary Medicine, Auburn University, AL, USA.
pubmed:publicationType
Journal Article, Research Support, N.I.H., Extramural