Source:http://linkedlifedata.com/resource/pubmed/id/21320495
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
5
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pubmed:dateCreated |
2011-3-4
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pubmed:abstractText |
Red fluorescent protein (RFP) DsRed and its variants are widely applied in live-cell imaging experiments. However, a major factor that restricts the application of DsRed is its cytotoxicity. Here, we report that DsRed and its variant DsRed-Express2 inhibit the expression of B-cell lymphoma-extra large (Bcl-xL) in HeLa cells by translational regulation. Over-expression of Bcl-xL can reduce the cytotoxicity of DsRed. Meanwhile, Turbo RFP, a mutant RFP from Entacmaea quadricolor, does not affect Bcl-xL expression, suggesting that cytotoxic mechanisms of RFP from different species may be varied. Our results reveal a possible mechanism for the cytotoxicity of DsRed, providing a potential strategy to improve the application of DsRed and its variants.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Green Fluorescent Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Luminescent Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Isoforms,
http://linkedlifedata.com/resource/pubmed/chemical/bcl-X Protein,
http://linkedlifedata.com/resource/pubmed/chemical/red fluorescent protein
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
1873-3468
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pubmed:author | |
pubmed:copyrightInfo |
Copyright © 2011 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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pubmed:issnType |
Electronic
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pubmed:day |
9
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pubmed:volume |
585
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
821-7
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pubmed:meshHeading |
pubmed-meshheading:21320495-Cell Death,
pubmed-meshheading:21320495-Down-Regulation,
pubmed-meshheading:21320495-Flow Cytometry,
pubmed-meshheading:21320495-Green Fluorescent Proteins,
pubmed-meshheading:21320495-HeLa Cells,
pubmed-meshheading:21320495-Humans,
pubmed-meshheading:21320495-Luminescent Proteins,
pubmed-meshheading:21320495-Microscopy, Fluorescence,
pubmed-meshheading:21320495-Protein Biosynthesis,
pubmed-meshheading:21320495-Protein Isoforms,
pubmed-meshheading:21320495-bcl-X Protein
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pubmed:year |
2011
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pubmed:articleTitle |
Cytotoxicity of red fluorescent protein DsRed is associated with the suppression of Bcl-xL translation.
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pubmed:affiliation |
Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Biomedical Engineering, Beihang University, China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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