Source:http://linkedlifedata.com/resource/pubmed/id/21317930
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
25
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pubmed:dateCreated |
2011-6-23
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pubmed:abstractText |
Axl is a receptor that induces proliferation, migration and invasion in cancer. In this study, we show that specific microRNAs (miRNAs) target the 3'-UTR of Axl. Luciferase-reporter assays with wild-type and deleted miR-34 and miR-199a/b seed sequences of Axl 3'-UTR confirmed the specificity of targeting. An inverse correlation between Axl protein and miR-34a expression in a panel of non-small cell lung cancer (NSCLC), colorectal cancer (CRC) and breast cancer (BRC) cell lines was observed, while miR-199a/b expression was completely suppressed. Pre-miR transfection inhibited in vitro migration and invasion and, in vivo, reduced the number of distant lung- or liver-metastases in a chorion-allantoic-membrane (CAM) assay. Moreover, methylation-specific PCR on bisulfite-converted DNA obtained from the cell lines showed that the miR-34a promoter methylation status was inversely correlated with its expression, and that miR-199a/b promoter regions were methylated in all cells tested. In a panel of NSCLC tissues (n=44), miR-34a and miR-199a/b were found to be downregulated and significantly co-expressed. A lower expression of all three miRs was significantly associated with squamous histotypes, and, in a preliminary series, NSCLC patients with miR-34a upregulation showed a positive association towards a longer survival. These results indicate that Axl receptor expression can be regulated by miR-34a and miR-199a/b, which are suppressed by promoter methylation in solid cancer cells.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/3' Untranslated Regions,
http://linkedlifedata.com/resource/pubmed/chemical/MIRN19 microRNA, human,
http://linkedlifedata.com/resource/pubmed/chemical/MIRN34 microRNA, human,
http://linkedlifedata.com/resource/pubmed/chemical/MicroRNAs,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor Protein-Tyrosine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/axl receptor tyrosine kinase
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
1476-5594
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pubmed:author | |
pubmed:issnType |
Electronic
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pubmed:day |
23
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pubmed:volume |
30
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2888-99
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pubmed:meshHeading |
pubmed-meshheading:21317930-3' Untranslated Regions,
pubmed-meshheading:21317930-Animals,
pubmed-meshheading:21317930-Base Sequence,
pubmed-meshheading:21317930-Cell Line, Tumor,
pubmed-meshheading:21317930-DNA Methylation,
pubmed-meshheading:21317930-Humans,
pubmed-meshheading:21317930-MicroRNAs,
pubmed-meshheading:21317930-Molecular Sequence Data,
pubmed-meshheading:21317930-Neoplasm Invasiveness,
pubmed-meshheading:21317930-Neoplasm Metastasis,
pubmed-meshheading:21317930-Neoplasms,
pubmed-meshheading:21317930-Promoter Regions, Genetic,
pubmed-meshheading:21317930-Proto-Oncogene Proteins,
pubmed-meshheading:21317930-Receptor Protein-Tyrosine Kinases,
pubmed-meshheading:21317930-Sequence Homology, Nucleic Acid
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pubmed:year |
2011
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pubmed:articleTitle |
Regulation of Axl receptor tyrosine kinase expression by miR-34a and miR-199a/b in solid cancer.
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pubmed:affiliation |
Department of Experimental Surgery and Molecular Oncology of Solid Tumors, University of Heidelberg, and German Cancer Research Center (DKFZ), Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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