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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
2011-3-28
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pubmed:abstractText |
Mouse brain expresses three principal glucose transporters. Glut1 is an endothelial marker and is the principal glucose transporter of the blood-brain barrier. Glut3 and Glut6 are expressed in glial cells and neural cells. A mouse line with a null allele for Glut3 has been developed. The Glut3(-/-) genotype is intrauterine lethal by 7days post-coitis, but the heterozygous (Glut3(+/-)) littermate survives, exhibiting rapid post-natal weight gain, but no seizures or other behavioral aberrations. At 12weeks of age, brain uptake of tail vein-injected ((3))H-2-deoxy glucose in Glut3(+/-) mice was not different from Glut3(+/+) littermates, despite 50% less Glut3 protein expression in the brain. The brain uptake of injected ((18))F-2-fluoro-2-deoxy glucose was similarly not different from Glut3(+/-) littermates in the total amount, time course, or brain imaging in the Glut3(+/-) mice. Glut1 and Glut6 protein expressions evaluated by immunoblots were not affected by the diminished Glut3 expression in the Glut3(+/-) mice. We conclude that a 50% decrease in Glut3 is not limiting for the uptake of glucose into the mouse brain, since Glut3 haploinsufficiency does not impair brain glucose uptake or utilization.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Blood Glucose,
http://linkedlifedata.com/resource/pubmed/chemical/Deoxyglucose,
http://linkedlifedata.com/resource/pubmed/chemical/Fluorodeoxyglucose F18,
http://linkedlifedata.com/resource/pubmed/chemical/Glucose,
http://linkedlifedata.com/resource/pubmed/chemical/Glucose Transporter Type 1,
http://linkedlifedata.com/resource/pubmed/chemical/Glucose Transporter Type 3,
http://linkedlifedata.com/resource/pubmed/chemical/Slc2a1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Slc2a3 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Tritium
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
1872-6240
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pubmed:author |
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pubmed:copyrightInfo |
Copyright © 2011 Elsevier B.V. All rights reserved.
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pubmed:issnType |
Electronic
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pubmed:day |
12
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pubmed:volume |
1384
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
15-22
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pubmed:meshHeading |
pubmed-meshheading:21316350-Analysis of Variance,
pubmed-meshheading:21316350-Animals,
pubmed-meshheading:21316350-Blood Glucose,
pubmed-meshheading:21316350-Brain,
pubmed-meshheading:21316350-Deoxyglucose,
pubmed-meshheading:21316350-Female,
pubmed-meshheading:21316350-Fluorodeoxyglucose F18,
pubmed-meshheading:21316350-Food Deprivation,
pubmed-meshheading:21316350-Gene Expression Regulation,
pubmed-meshheading:21316350-Glucose,
pubmed-meshheading:21316350-Glucose Transporter Type 1,
pubmed-meshheading:21316350-Glucose Transporter Type 3,
pubmed-meshheading:21316350-Male,
pubmed-meshheading:21316350-Mice,
pubmed-meshheading:21316350-Mice, Inbred C57BL,
pubmed-meshheading:21316350-Mice, Knockout,
pubmed-meshheading:21316350-Positron-Emission Tomography,
pubmed-meshheading:21316350-Tomography, Emission-Computed, Single-Photon,
pubmed-meshheading:21316350-Tritium
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pubmed:year |
2011
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pubmed:articleTitle |
Brain glucose transporter (Glut3) haploinsufficiency does not impair mouse brain glucose uptake.
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pubmed:affiliation |
East Tennessee State University Quillen College of Medicine, Johnson City, TN, USA. stuartc@etsu.edu
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pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Extramural
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