Linked Life Data

21315800

Source:http://linkedlifedata.com/resource/pubmed/id/21315800

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PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
2011-4-8
pubmed:abstractText
The higher prevalence of thyroid disease in women suggests that estrogen (E2) might be involved in the pathophysiology of thyroid dysfunction. To approach the question of the effect of stromal cells in the modulation of thyroid epithelial cells activity, we established and characterized a homogeneous stromal cell population (TS7 cells) of rat thyroid gland. These fibroblastic cells synthesize the cytoskeleton proteins ?-smooth muscle actin and vimentin, produce basement membrane components and express the cytokine transforming growth factor beta 1 (TGF-?1). Here, we hypothesized that the effects of E2 on follicular thyroid cells are mediated by TGF-?1 synthesis and secretion by stromal cells (paracrine action). Thus we investigated the effect of E2 on TGF-?1 synthesis and its signaling pathway in TS7 cells. In addition, we analyzed the role of TGF-?1 signaling pathway as mediator of TS7-PC CL3 thyroid epithelial cells interactions. We report that TS7 stromal cells expressed ? and ? estrogen receptors (ER? and ER?). Further, both isoforms of TGF-?1 receptors, TGFRI and TGFRII, were also identified in TS7 cells, suggesting that these cells might be a target for this cytokine in vitro. Treatment of TS7 cells with E2 induced both synthesis and secretion of TGF-?1. This event was followed by phosphorylation of the transcription factor Smad2, a hallmark of TGF-?1 pathway activation. Co-culture of PC CL3 cells onto TS7 cells monolayers yielded round aggregates of PC CL3 cells surrounded by TS7 cells. TS7 cells induced a decrease in iodide uptake by PC CL3 cells, probably by a mechanism involving TGF-?1. Moreover, E2 affected synthesis and organization of the extracellular matrix (ECM) components, tenascin C and chondroitin sulfate, in these co-culture cells. Our results point to the TGF-?1/Smad-2 signaling pathway as a putative target of estrogen actions on thyroid stromal cells and contribute to understanding the interplay between stromal and follicular cells in thyroid physiology.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1872-8057
pubmed:author
pubmed:copyrightInfo
Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.
pubmed:issnType
Electronic
pubmed:day
30
pubmed:volume
337
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
71-9
pubmed:meshHeading
pubmed-meshheading:21315800-Animals, pubmed-meshheading:21315800-Cell Shape, pubmed-meshheading:21315800-Cell Survival, pubmed-meshheading:21315800-Cells, Cultured, pubmed-meshheading:21315800-Coculture Techniques, pubmed-meshheading:21315800-Cytoskeletal Proteins, pubmed-meshheading:21315800-Estradiol, pubmed-meshheading:21315800-Estrogen Receptor alpha, pubmed-meshheading:21315800-Estrogen Receptor beta, pubmed-meshheading:21315800-Extracellular Matrix, pubmed-meshheading:21315800-Female, pubmed-meshheading:21315800-Rats, pubmed-meshheading:21315800-Rats, Wistar, pubmed-meshheading:21315800-Receptors, Transforming Growth Factor beta, pubmed-meshheading:21315800-Signal Transduction, pubmed-meshheading:21315800-Smad Proteins, pubmed-meshheading:21315800-Stromal Cells, pubmed-meshheading:21315800-Thyroid Gland, pubmed-meshheading:21315800-Transcription, Genetic, pubmed-meshheading:21315800-Transforming Growth Factor beta1
pubmed:year
2011
pubmed:articleTitle
Estradiol modulates TGF-?1 expression and its signaling pathway in thyroid stromal cells.
pubmed:affiliation
Laboratory of Cellular Interactions, Program of Cellular Biology and Development, Institute of Biomedical Sciences, Federal University of Rio de Janeiro, Brazil.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't