Linked Life Data

21312047

Source:http://linkedlifedata.com/resource/pubmed/id/21312047

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2011-3-22
pubmed:abstractText
There is evidence to suggest that low levels of magnesium (Mg) are associated with affective disorders, however, causality and central neurobiological mechanisms of this link are largely unproven. We have recently shown that mice fed a low Mg-containing diet (10% of daily requirement) display enhanced depression-like behavior sensitive to chronic antidepressant treatment. The aim of the present study was to utilize this model to gain insight into underlying mechanisms by quantifying amygdala/hypothalamus protein expression using gel-based proteomics and correlating changes in protein expression with changes in depression-like behavior. Mice fed Mg-restricted diet displayed reduced brain Mg tissue levels and altered expression of four proteins, N(G),N(G)-dimethylarginine dimethylaminohydrolase 1 (DDAH1), manganese-superoxide dismutase (MnSOD), glutamate dehydrogenase 1 (GDH1) and voltage-dependent anion channel 1. The observed alterations in protein expression may indicate increased nitric oxide production, increased anti-oxidant response to increased oxidative stress and potential alteration in energy metabolism. Aberrant expressions of DDAH1, MnSOD and GDH1 were normalized by chronic paroxetine treatment which also normalized the enhanced depression-like behavior, strengthening the link between the changes in these proteins and depression-like behavior. Collectively, these findings provide first evidence of low magnesium-induced alteration in brain protein levels and biochemical pathways, contributing to central dysregulation in affective disorders.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1438-2199
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
40
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1231-48
pubmed:meshHeading
pubmed-meshheading:21312047-Amidohydrolases, pubmed-meshheading:21312047-Amygdala, pubmed-meshheading:21312047-Animals, pubmed-meshheading:21312047-Antidepressive Agents, pubmed-meshheading:21312047-Depression, pubmed-meshheading:21312047-Diet, pubmed-meshheading:21312047-Electrophoresis, Gel, Two-Dimensional, pubmed-meshheading:21312047-Gene Expression Profiling, pubmed-meshheading:21312047-Glutamate Dehydrogenase, pubmed-meshheading:21312047-Hypothalamus, pubmed-meshheading:21312047-Magnesium Deficiency, pubmed-meshheading:21312047-Male, pubmed-meshheading:21312047-Mice, pubmed-meshheading:21312047-Mice, Inbred C57BL, pubmed-meshheading:21312047-Nitric Oxide, pubmed-meshheading:21312047-Oxidative Stress, pubmed-meshheading:21312047-Paroxetine, pubmed-meshheading:21312047-Proteomics, pubmed-meshheading:21312047-Superoxide Dismutase, pubmed-meshheading:21312047-Tandem Mass Spectrometry, pubmed-meshheading:21312047-Voltage-Dependent Anion Channel 1
pubmed:year
2011
pubmed:articleTitle
Changes in brain protein expression are linked to magnesium restriction-induced depression-like behavior.
pubmed:affiliation
Department of Pharmacology and Toxicology and Center for Molecular Biosciences, University of Innsbruck, Innsbruck, Austria.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't