|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
2
|
|
pubmed:dateCreated |
2011-2-11
|
|
pubmed:abstractText |
Time-domain Fluorescence Lifetime Imaging Microscopy (FLIM) is a remarkable tool to monitor the dynamics of fluorophore-tagged protein domains inside living cells. We propose a Wide-Field Multi-Parameter FLIM method (WFMP-FLIM) aimed to monitor continuously living cells under minimum light intensity at a given illumination energy dose. A powerful data analysis technique applied to the WFMP-FLIM data sets allows to optimize the estimation accuracy of physical parameters at very low fluorescence signal levels approaching the lower bound theoretical limit. We demonstrate the efficiency of WFMP-FLIM by presenting two independent and relevant long-term experiments in cell biology: 1) FRET analysis of simultaneously recorded donor and acceptor fluorescence in living HeLa cells and 2) tracking of mitochondrial transport combined with fluorescence lifetime analysis in neuronal processes.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/21311595-10087617,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21311595-10733990,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21311595-11209760,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21311595-11354494,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21311595-12001993,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21311595-12067366,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21311595-12496124,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21311595-12496126,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21311595-14535891,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21311595-14595367,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21311595-15175430,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21311595-15312193,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21311595-15454472,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21311595-15883770,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21311595-16113120,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21311595-16306220,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21311595-16704336,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21311595-16896339,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21311595-16902090,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21311595-16949332,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21311595-17269654,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21311595-17336446,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21311595-17572680,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21311595-17904380,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21311595-17933880,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21311595-18454154,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21311595-18665222,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21311595-19097172,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21311595-19548231,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21311595-19693494,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21311595-20006503,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21311595-20431544,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21311595-20490396,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21311595-20643879,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21311595-9591694
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:issn |
1932-6203
|
|
pubmed:author |
|
|
pubmed:issnType |
Electronic
|
|
pubmed:volume |
6
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
e15820
|
|
pubmed:meshHeading |
pubmed-meshheading:21311595-Algorithms,
pubmed-meshheading:21311595-Biological Transport,
pubmed-meshheading:21311595-Cells,
pubmed-meshheading:21311595-Efficiency,
pubmed-meshheading:21311595-Fluorescence Resonance Energy Transfer,
pubmed-meshheading:21311595-Green Fluorescent Proteins,
pubmed-meshheading:21311595-HeLa Cells,
pubmed-meshheading:21311595-Humans,
pubmed-meshheading:21311595-Microscopy, Fluorescence,
pubmed-meshheading:21311595-Mitochondria,
pubmed-meshheading:21311595-Observation,
pubmed-meshheading:21311595-Photobleaching,
pubmed-meshheading:21311595-Proteins,
pubmed-meshheading:21311595-Single-Cell Analysis,
pubmed-meshheading:21311595-Time Factors,
pubmed-meshheading:21311595-Time-Lapse Imaging
|
|
pubmed:year |
2011
|
|
pubmed:articleTitle |
Wide-Field Multi-Parameter FLIM: long-term minimal invasive observation of proteins in living cells.
|
|
pubmed:affiliation |
Leibniz Institute for Neurobiology, Magdeburg, Germany.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Evaluation Studies
|
