Linked Life Data

2131099

Source:http://linkedlifedata.com/resource/pubmed/id/2131099

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1991-12-6
pubmed:abstractText
A strain of tobacco mosaic virus (TMV) that produces mild (attenuated) symptoms on tobacco plants has been molecularly cloned to identify the region of the genome responsible for symptom attenuation. A full-length cDNA clone whose transcripts produce the parental disease phenotype on both systemic and hypersensitive host plants has been constructed. This infectious clone was sequenced, and 55 base changes relative to the published sequence of common TMV (strain U1) were identified. These changes resulted in 12 amino acid alterations in the open reading frames encoding the 126/183-kDa and 30-kDa movement proteins; two of these changes were determined not to be responsible for the attenuated phenotype. Exchange of restriction fragments between the infectious mild strain cDNA and an infectious U1 strain cDNA indicated that the determinants involved in symptom attenuation reside in the open reading frame encoding the 126/183-kDa proteins of TMV; these proteins are involved in viral replication.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0894-0282
pubmed:author
pubmed:issnType
Print
pubmed:volume
3
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
417-23
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:articleTitle
Characterization of the masked strain of tobacco mosaic virus: identification of the region responsible for symptom attenuation by analysis of an infectious cDNA clone.
pubmed:affiliation
Department of Biology, Washington University, St. Louis, MO 63130.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't