Source:http://linkedlifedata.com/resource/pubmed/id/21310868
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
4
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pubmed:dateCreated |
2011-3-22
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pubmed:abstractText |
Because dietary arachidonate (ARA) and its eicosanoid derivatives are major regulators of intestinal homeostasis and repair following injury, we evaluated the effects of dietary ARA on desaturation and elongation of (13)C-18:2(n-6) and mRNA abundance of ?-6-desaturase (FADS2), elongase (ELOVL5), and ?-5-desaturase (FADS1) in liver and intestine. Day-old pigs (n = 96) were fed milk-based formula containing 0, 0.5, 2.5, or 5% ARA or 5% eicosapentaenoic acid of total fatty acids for 4, 8, and 16 d. In liver, the desaturation rate [nmol/(g tissue?h)] of (13)C-18:2(n-6) to (13)C-18:3(n-6) decreased 56% between 4 and 16 d but was not affected by diet. Whereas accumulation in (13)C-20:3(n-6) also decreased with age by 67%, it increased linearly with increasing dietary ARA (P < 0.06). In comparison, intestinal flux was ~50% less than liver flux and was unaffected by age, but desaturation to (13)C-18:3(n-6) increased linearly (by 57%) in pigs fed ARA diets (P < 0.001), equaling the rate observed in sow-fed controls. In both liver and intestine, alternate elongation to (13)C-20:2(n-6) (via ?-8-desaturase) was markedly elevated in pigs fed the 0% ARA diet compared with all other dietary treatments (P < 0.01). Transcript abundance of FADS2, ELOVL5, and FADS1 was not affected in liver by diet (P > 0.05) but decreased precipitously between birth and d 4 (~70%; P < 0.05). In contrast, intestinal abundance of FADS2 and FADS1 increased 60% from d 4 to 16. In conclusion, dietary ARA regulated the desaturase-elongase pathway in a tissue-specific manner. In liver, ARA had modest effects on (n-6) fatty acid flux, and intestinal FADS2 activity and mRNA increased. Additionally, hepatic flux decreased with postnatal age, whereas intestinal flux did not change.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Acetyltransferases,
http://linkedlifedata.com/resource/pubmed/chemical/Arachidonic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Fatty Acid Desaturases,
http://linkedlifedata.com/resource/pubmed/chemical/Fatty Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Linoleoyl-CoA Desaturase,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/delta-5 fatty acid desaturase,
http://linkedlifedata.com/resource/pubmed/chemical/fatty acid elongases
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
1541-6100
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pubmed:author | |
pubmed:issnType |
Electronic
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pubmed:day |
1
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pubmed:volume |
141
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
548-53
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pubmed:meshHeading |
pubmed-meshheading:21310868-Acetyltransferases,
pubmed-meshheading:21310868-Animals,
pubmed-meshheading:21310868-Animals, Suckling,
pubmed-meshheading:21310868-Arachidonic Acid,
pubmed-meshheading:21310868-Fatty Acid Desaturases,
pubmed-meshheading:21310868-Fatty Acids,
pubmed-meshheading:21310868-Intestines,
pubmed-meshheading:21310868-Linoleoyl-CoA Desaturase,
pubmed-meshheading:21310868-Liver,
pubmed-meshheading:21310868-Organ Specificity,
pubmed-meshheading:21310868-Protein Transport,
pubmed-meshheading:21310868-RNA, Messenger,
pubmed-meshheading:21310868-Swine
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pubmed:year |
2011
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pubmed:articleTitle |
Dietary arachidonate differentially alters desaturase-elongase pathway flux and gene expression in liver and intestine of suckling pigs.
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pubmed:affiliation |
Laboratory of Developmental Nutrition, Department of Animal Science, North Carolina State University, Raleigh, NC 27695, USA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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