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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
2011-5-17
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pubmed:abstractText |
Epithelial invagination in many model systems is driven by apical cell constriction, mediated by actin and myosin II contraction regulated by GTPase activity. Here we investigate apical constriction during chick lens placode invagination. Inhibition of actin polymerization and myosin II activity by cytochalasin D or blebbistatin prevents lens invagination. To further verify if lens placode invaginate through apical constriction, we analyzed the role of Rho-ROCK pathway. Rho GTPases expression at the apical portion of the lens placode occurs with the same dynamics as that of the cytoskeleton. Overexpression of the pan-Rho inhibitor C3 exotoxin abolished invagination and had a strong effect on apical myosin II enrichment and a mild effect on apical actin localization. In contrast, pharmacological inhibition of ROCK activity interfered significantly with apical enrichment of both actin and myosin. These results suggest that apical constriction in lens invagination involves ROCK but apical concentration of actin and myosin are regulated through different pathways upstream of ROCK. genesis 49:368-379, 2011.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/ADP Ribose Transferases,
http://linkedlifedata.com/resource/pubmed/chemical/Actins,
http://linkedlifedata.com/resource/pubmed/chemical/Actomyosin,
http://linkedlifedata.com/resource/pubmed/chemical/Amides,
http://linkedlifedata.com/resource/pubmed/chemical/Botulinum Toxins,
http://linkedlifedata.com/resource/pubmed/chemical/Cytochalasin D,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Heterocyclic Compounds with 4 or...,
http://linkedlifedata.com/resource/pubmed/chemical/Myosin Type II,
http://linkedlifedata.com/resource/pubmed/chemical/Nucleic Acid Synthesis Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Pyridines,
http://linkedlifedata.com/resource/pubmed/chemical/Y 27632,
http://linkedlifedata.com/resource/pubmed/chemical/blebbistatin,
http://linkedlifedata.com/resource/pubmed/chemical/exoenzyme C3, Clostridium botulinum,
http://linkedlifedata.com/resource/pubmed/chemical/rho GTP-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/rho-Associated Kinases
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
1526-968X
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pubmed:author |
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pubmed:copyrightInfo |
2011 Wiley-Liss, Inc.
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pubmed:issnType |
Electronic
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pubmed:volume |
49
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
368-79
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pubmed:meshHeading |
pubmed-meshheading:21309072-ADP Ribose Transferases,
pubmed-meshheading:21309072-Actins,
pubmed-meshheading:21309072-Actomyosin,
pubmed-meshheading:21309072-Amides,
pubmed-meshheading:21309072-Animals,
pubmed-meshheading:21309072-Botulinum Toxins,
pubmed-meshheading:21309072-Chick Embryo,
pubmed-meshheading:21309072-Chickens,
pubmed-meshheading:21309072-Cytochalasin D,
pubmed-meshheading:21309072-Cytoskeleton,
pubmed-meshheading:21309072-Ectoderm,
pubmed-meshheading:21309072-Enzyme Inhibitors,
pubmed-meshheading:21309072-Female,
pubmed-meshheading:21309072-Fluorescent Antibody Technique,
pubmed-meshheading:21309072-Heterocyclic Compounds with 4 or More Rings,
pubmed-meshheading:21309072-Immunohistochemistry,
pubmed-meshheading:21309072-Lens, Crystalline,
pubmed-meshheading:21309072-Myosin Type II,
pubmed-meshheading:21309072-Nucleic Acid Synthesis Inhibitors,
pubmed-meshheading:21309072-Pyridines,
pubmed-meshheading:21309072-Signal Transduction,
pubmed-meshheading:21309072-rho GTP-Binding Proteins,
pubmed-meshheading:21309072-rho-Associated Kinases
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pubmed:year |
2011
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pubmed:articleTitle |
Rho signaling pathway and apical constriction in the early lens placode.
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pubmed:affiliation |
Department of Cell and Developmental Biology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, SP, Brazil.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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