Linked Life Data

21302221

Source:http://linkedlifedata.com/resource/pubmed/id/21302221

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2011-2-8
pubmed:abstractText
We previously reported gene therapy using cationized gelatin microspheres of ?20-32 ?m, prepared from pig skin, as a transducing agent, but although the gelatin offered various advantages, its yield was extremely low (only 0.1%). In this study, we markedly improved the yield of ?20-32 ?m cationized gelatin microspheres and prepared a newly less than ?20 ?m cationized gelatin. Conventionally, cationized gelatin is prepared by cationization, particulation by agitation, and cross-linking. The yield is determined by the particulation step, for which we had used a three-necked distillation flask of 500 mL and an agitation speed of 420 rpm. The yield was significantly increased from 0.13 ± 0.02% to 8.80 ± 1.90% by using a smaller flask of 300 mL and an agitation speed of 25000 rpm (p ? 0.01). We could also prepare cationized gelatin of less than ?20 ?m, which had not been possible previously. We confirmed that efficient gene introduction into peritoneal macrophages could be achieved with the new cationized gelatin.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
2185-2243
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
31
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
49-52
pubmed:meshHeading
pubmed:year
2006
pubmed:articleTitle
Efficient preparation of cationized gelatin for gene transduction.
pubmed:affiliation
Department of Physiology, Tokai University, Bohseidai, Isehara, Kanagawa 259-1193, Japan. fukuyama@is.icc.u-tokai.ac.jp
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't