Linked Life Data

21297037

Source:http://linkedlifedata.com/resource/pubmed/id/21297037

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2011-3-28
pubmed:abstractText
Following the endosymbiotic acquisition of mitochondria by eukaryotic cells, most of the genes in this organelle were transferred to the nucleus. To maintain mitochondrial biogenesis and function, nuclear and mitochondrial genomes require regulated and coordinated expression. In plant organelles, nuclear-encoded proteins targeted to the organelles control posttranscriptional and posttranslational mechanisms. Pentatricopeptide repeat (PPR) proteins are good candidates to play such regulatory roles. Here, we identify PNM1 (for PPR protein localized to the nucleus and mitochondria 1), a novel PPR protein that is dual localized to mitochondria and nuclei in Arabidopsis thaliana, as observed by green fluorescent protein fusions and immunodetection on subcellular fractions and on histological sections. Genetic complementation showed that loss of PNM1 function in mitochondria, but not in nuclei, is lethal for the embryo. In mitochondria, it is associated with polysomes and may play a role in translation. A genetic screen in yeast identified protein partners of PNM1. These partners, the nucleosome assembly protein NAP1, and the transcription factor TCP8 interact with PNM1 in the nucleus in planta. Furthermore, TCP8 can bind the promoter of PNM1. This suggests that PNM1 might be involved in the regulation of its own gene expression in the nucleus and could thus play a role in gene expression adjustments between mitochondria and the nucleus.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1532-298X
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
23
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
730-40
pubmed:meshHeading
pubmed-meshheading:21297037-Adenosine Triphosphatases, pubmed-meshheading:21297037-Arabidopsis, pubmed-meshheading:21297037-Arabidopsis Proteins, pubmed-meshheading:21297037-Gene Expression Regulation, Developmental, pubmed-meshheading:21297037-Gene Expression Regulation, Plant, pubmed-meshheading:21297037-Genes, Lethal, pubmed-meshheading:21297037-Genetic Complementation Test, pubmed-meshheading:21297037-Mitochondria, pubmed-meshheading:21297037-Mitochondrial Proteins, pubmed-meshheading:21297037-Mutation, pubmed-meshheading:21297037-Nuclear Proteins, pubmed-meshheading:21297037-Polyribosomes, pubmed-meshheading:21297037-Promoter Regions, Genetic, pubmed-meshheading:21297037-Protein Interaction Mapping, pubmed-meshheading:21297037-RNA, Plant, pubmed-meshheading:21297037-RNA-Binding Proteins, pubmed-meshheading:21297037-Transcription Factors
pubmed:year
2011
pubmed:articleTitle
An Arabidopsis dual-localized pentatricopeptide repeat protein interacts with nuclear proteins involved in gene expression regulation.
pubmed:affiliation
Institut de Biologie Moléculaire des Plantes du Centre National de la Recherche Scientifique, Université de Strasbourg, 67084 Strasbourg Cedex, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't