Source:http://linkedlifedata.com/resource/pubmed/id/21295561
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
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| pubmed:dateCreated |
2011-3-28
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| pubmed:abstractText |
Branching morphogenesis in murine submandibular glands (SMG) is regulated by growth factors, extracellular matrix (ECM) and many biological processes through interactions between the epithelium and the mesenchyme. MicroRNAs (miRNAs) are a set of small, non-protein-coding RNAs that regulate gene expression at the post-transcriptional level. We hypothesized that branching morphogenesis is partly regulated by miRNAs. Forty-four miRNAs and novel miRNA candidates were detected in SMG at embryonic day 13 by a cloning method combined with Argonaute-2 immunoprecipitation. MicroRNA21 (miR-21) expression in the mesenchyme was up-regulated and accelerated by epidermal growth factor, which is known to enhance branching morphogenesis in vitro. Down-regulation of miR-21 in the mesenchyme by locked nucleic acids was associated with a decrease in the number of epithelial buds. Relative quantification of candidates for target genes of miR-21 indicated that two messenger RNAs (for Reck and Pdcd4) were down-regulated in the mesenchyme, where miR-21 expression levels were up-regulated. These results suggest that branching morphogenesis is regulated by miR-21 through gene expression related to ECM degradation in the mesenchyme.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/Epidermal Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/MIRN21 microRNA, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/MicroRNAs,
http://linkedlifedata.com/resource/pubmed/chemical/Oligonucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/locked nucleic acid
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| pubmed:status |
MEDLINE
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| pubmed:month |
Apr
|
| pubmed:issn |
1095-564X
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| pubmed:author | |
| pubmed:copyrightInfo |
Copyright © 2011 Elsevier Inc. All rights reserved.
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| pubmed:issnType |
Electronic
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| pubmed:day |
15
|
| pubmed:volume |
352
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
299-307
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| pubmed:meshHeading |
pubmed-meshheading:21295561-Animals,
pubmed-meshheading:21295561-Base Sequence,
pubmed-meshheading:21295561-DNA Primers,
pubmed-meshheading:21295561-Down-Regulation,
pubmed-meshheading:21295561-Epidermal Growth Factor,
pubmed-meshheading:21295561-Extracellular Matrix,
pubmed-meshheading:21295561-Gene Expression Regulation, Developmental,
pubmed-meshheading:21295561-Mesoderm,
pubmed-meshheading:21295561-Mice,
pubmed-meshheading:21295561-Mice, Inbred ICR,
pubmed-meshheading:21295561-MicroRNAs,
pubmed-meshheading:21295561-Morphogenesis,
pubmed-meshheading:21295561-Nucleic Acid Conformation,
pubmed-meshheading:21295561-Oligonucleotides,
pubmed-meshheading:21295561-Sequence Homology, Nucleic Acid,
pubmed-meshheading:21295561-Submandibular Gland,
pubmed-meshheading:21295561-Transfection,
pubmed-meshheading:21295561-Up-Regulation
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| pubmed:year |
2011
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| pubmed:articleTitle |
Mesenchymal miR-21 regulates branching morphogenesis in murine submandibular gland in vitro.
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| pubmed:affiliation |
Department of Pharmacology, Asahi University School of Dentistry, 1851 Hozumi, Mizuho, Gifu 501–0296, Japan.
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| pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
|