Source:http://linkedlifedata.com/resource/pubmed/id/21293464
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
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| pubmed:dateCreated |
2011-3-10
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| pubmed:abstractText |
Human induced pluripotent stem cells (iPSCs) present exciting opportunities for studying development and for in vitro disease modeling. However, reported variability in the behavior of iPSCs has called their utility into question. We established a test set of 16 iPSC lines from seven individuals of varying age, sex and health status, and extensively characterized the lines with respect to pluripotency and the ability to terminally differentiate. Under standardized procedures in two independent laboratories, 13 of the iPSC lines gave rise to functional motor neurons with a range of efficiencies similar to that of human embryonic stem cells (ESCs). Although three iPSC lines were resistant to neural differentiation, early neuralization rescued their performance. Therefore, all 16 iPSC lines passed a stringent test of differentiation capacity despite variations in karyotype and in the expression of early pluripotency markers and transgenes. This iPSC and ESC test set is a robust resource for those interested in the basic biology of stem cells and their applications.
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| pubmed:grant | |
| pubmed:commentsCorrections | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
1546-1696
|
| pubmed:author |
pubmed-author:AmorosoMackenzie WMW,
pubmed-author:BoultingGabriella LGL,
pubmed-author:CroftGist FGF,
pubmed-author:DavidowLanceL,
pubmed-author:DimosJohn TJT,
pubmed-author:EgganKevinK,
pubmed-author:HendersonChristopher ECE,
pubmed-author:KahlerDavid JDJ,
pubmed-author:KiskinisEvangelosE,
pubmed-author:MacDermottAmy BAB,
pubmed-author:MikkilineniShravaniS,
pubmed-author:OakleyDerek HDH,
pubmed-author:RodolfaChristopher TCT,
pubmed-author:WaingerBrian JBJ,
pubmed-author:WichterleHynekH,
pubmed-author:WilliamsDamian JDJ,
pubmed-author:WoolfClifford JCJ,
pubmed-author:YamakiMarikoM
|
| pubmed:issnType |
Electronic
|
| pubmed:volume |
29
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
279-86
|
| pubmed:dateRevised |
2011-9-22
|
| pubmed:meshHeading |
pubmed-meshheading:21293464-Cell Culture Techniques,
pubmed-meshheading:21293464-Cell Differentiation,
pubmed-meshheading:21293464-Cells, Cultured,
pubmed-meshheading:21293464-Fibroblasts,
pubmed-meshheading:21293464-Humans,
pubmed-meshheading:21293464-Pluripotent Stem Cells,
pubmed-meshheading:21293464-Skin,
pubmed-meshheading:21293464-Tissue Engineering
|
| pubmed:year |
2011
|
| pubmed:articleTitle |
A functionally characterized test set of human induced pluripotent stem cells.
|
| pubmed:affiliation |
The Howard Hughes Medical Institute, Cambridge, Massachusetts, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
|