Linked Life Data

21291382

Source:http://linkedlifedata.com/resource/pubmed/id/21291382

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2011-4-27
pubmed:abstractText
The archaeolipids (lipids extracted from archaebacterias) are non saponificable molecules that form self sealed mono or bilayers (archaeosomes-ARC). Different to liposomes with bilayers made of conventional glycerophospholipids, the bilayer of ARC posses a higher structural resistance to physico chemical and enzymatic degradation and surface hydrophobicity. In this work we have compared the binding capacity of ARC exclusively made of archaeols containing a minor fraction of sulphoglycophospholipids, with that of liposomes in gel phase on M-like cells in vitro. The biodistribution of the radiopharmaceutical (99m)Tc-DTPA loaded in ARC vs that of liposomes upon oral administration to Wistar rats was also determined. The fluorescence of M-like cells upon 1 and 2h incubation with ARC loaded with the hydrophobic dye Rhodamine-PE (Rh-PE) and the hydrophilic dye pyranine (HPTS) dissolved in the aqueous space, was 4 folds higher than upon incubation with equally labeled liposomes. Besides, 15% of Rh-PE and 13 % of HPTS from ARC and not from liposomes, were found in the bottom wells, a place that is equivalent to the basolateral pocket from M cells. This fact suggested the occurrence of transcytosis of ARC. Finally, 4 h upon oral administration, ARC were responsible for the 22.3 % (3.5 folds higher than liposomes) shuttling of (99m)Tc-DTPA to the blood circulation. This important amount of radioactive marker in blood could be a consequence of an extensive uptake of ARC by M cells in vivo, probably favored by their surface hydrophobicity. Taken together, these results suggested that ARC, proven their adjuvant capacity when administered by parenteral route and high biocompatibility, could be a suitable new type of nanoparticulate material that could be used as adjuvants by the oral route.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1875-5704
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
8
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
320-9
pubmed:dateRevised
2011-9-29
pubmed:meshHeading
pubmed-meshheading:21291382-Administration, Oral, pubmed-meshheading:21291382-Animals, pubmed-meshheading:21291382-Archaea, pubmed-meshheading:21291382-Arylsulfonates, pubmed-meshheading:21291382-Caco-2 Cells, pubmed-meshheading:21291382-Cell Line, Tumor, pubmed-meshheading:21291382-Coculture Techniques, pubmed-meshheading:21291382-Drug Carriers, pubmed-meshheading:21291382-Glyceryl Ethers, pubmed-meshheading:21291382-Humans, pubmed-meshheading:21291382-Hydrophobic and Hydrophilic Interactions, pubmed-meshheading:21291382-Liposomes, pubmed-meshheading:21291382-Nanoparticles, pubmed-meshheading:21291382-Rats, pubmed-meshheading:21291382-Rats, Wistar, pubmed-meshheading:21291382-Rhodamines, pubmed-meshheading:21291382-Technetium Tc 99m Pentetate, pubmed-meshheading:21291382-Tissue Distribution, pubmed-meshheading:21291382-Transcytosis
pubmed:year
2011
pubmed:articleTitle
M cells prefer archaeosomes: an in vitro/in vivo snapshot upon oral gavage in rats.
pubmed:affiliation
Programa de Nanomedicinas, Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmas, Roque Saenz Peña 352, Bernal, B1876 BXD, Buenos Aires, Argentina.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't