Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2011-2-3
pubmed:abstractText
Many auditory, vestibular, and lateral-line afferent neurons display spontaneous action potentials. This spontaneous spiking is thought to result from hair-cell glutamate release in the absence of stimuli. Spontaneous release at hair-cell resting potentials presumably results from Ca(V)1.3 L-type calcium channel activity. Here, using intact zebrafish larvae, we recorded robust spontaneous spiking from lateral-line afferent neurons in the absence of external stimuli. Consistent with the above assumptions, spiking was absent in mutants that lacked either Vesicular glutamate transporter 3 (Vglut3) or Ca(V)1.3. We then tested the hypothesis that spontaneous spiking resulted from sustained Ca(V)1.3 activity due to depolarizing currents that are active at rest. Mechanotransduction currents (I(MET)) provide a depolarizing influence to the resting potential. However, following block of I(MET), spontaneous spiking persisted and was characterized by longer interspike intervals and increased periods of inactivity. These results suggest that an additional depolarizing influence maintains the resting potential within the activation range of Ca(V)1.3. To test whether the hyperpolarization-activated cation current, I(h) participates in setting the resting potential, we applied I(h) antagonists. Both ZD7288 and DK-AH 269 reduced spontaneous activity. Finally, concomitant block of I(MET) and I(h) essentially abolished spontaneous activity, ostensibly by hyperpolarization outside of the activation range for Ca(V)1.3. Together, our data support a mechanism for spontaneous spiking that results from Ca(2+)-dependent neurotransmitter release at hair-cell resting potentials that are maintained within the activation range of Ca(V)1.3 channels through active I(MET) and I(h).
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/21289170-10414361, http://linkedlifedata.com/resource/pubmed/commentcorrection/21289170-10526320, http://linkedlifedata.com/resource/pubmed/commentcorrection/21289170-10639174, http://linkedlifedata.com/resource/pubmed/commentcorrection/21289170-10651007, http://linkedlifedata.com/resource/pubmed/commentcorrection/21289170-10712472, http://linkedlifedata.com/resource/pubmed/commentcorrection/21289170-10831861, http://linkedlifedata.com/resource/pubmed/commentcorrection/21289170-11559767, http://linkedlifedata.com/resource/pubmed/commentcorrection/21289170-11588185, http://linkedlifedata.com/resource/pubmed/commentcorrection/21289170-11728644, http://linkedlifedata.com/resource/pubmed/commentcorrection/21289170-11929895, http://linkedlifedata.com/resource/pubmed/commentcorrection/21289170-12037175, http://linkedlifedata.com/resource/pubmed/commentcorrection/21289170-12093909, http://linkedlifedata.com/resource/pubmed/commentcorrection/21289170-12466445, http://linkedlifedata.com/resource/pubmed/commentcorrection/21289170-12740421, http://linkedlifedata.com/resource/pubmed/commentcorrection/21289170-12805553, http://linkedlifedata.com/resource/pubmed/commentcorrection/21289170-13175199, http://linkedlifedata.com/resource/pubmed/commentcorrection/21289170-13945569, http://linkedlifedata.com/resource/pubmed/commentcorrection/21289170-14645476, http://linkedlifedata.com/resource/pubmed/commentcorrection/21289170-14759575, http://linkedlifedata.com/resource/pubmed/commentcorrection/21289170-15056680, http://linkedlifedata.com/resource/pubmed/commentcorrection/21289170-15057246, http://linkedlifedata.com/resource/pubmed/commentcorrection/21289170-15115817, http://linkedlifedata.com/resource/pubmed/commentcorrection/21289170-15157418, http://linkedlifedata.com/resource/pubmed/commentcorrection/21289170-15181168, http://linkedlifedata.com/resource/pubmed/commentcorrection/21289170-15634702, http://linkedlifedata.com/resource/pubmed/commentcorrection/21289170-15781896, http://linkedlifedata.com/resource/pubmed/commentcorrection/21289170-16266369, http://linkedlifedata.com/resource/pubmed/commentcorrection/21289170-16285850, http://linkedlifedata.com/resource/pubmed/commentcorrection/21289170-16354915, http://linkedlifedata.com/resource/pubmed/commentcorrection/21289170-16567618, http://linkedlifedata.com/resource/pubmed/commentcorrection/21289170-16828974, http://linkedlifedata.com/resource/pubmed/commentcorrection/21289170-17135414, http://linkedlifedata.com/resource/pubmed/commentcorrection/21289170-1764247, http://linkedlifedata.com/resource/pubmed/commentcorrection/21289170-17670993, http://linkedlifedata.com/resource/pubmed/commentcorrection/21289170-17972875, http://linkedlifedata.com/resource/pubmed/commentcorrection/21289170-18174213, http://linkedlifedata.com/resource/pubmed/commentcorrection/21289170-18305245, http://linkedlifedata.com/resource/pubmed/commentcorrection/21289170-18322004, http://linkedlifedata.com/resource/pubmed/commentcorrection/21289170-18716202, http://linkedlifedata.com/resource/pubmed/commentcorrection/21289170-19223970, http://linkedlifedata.com/resource/pubmed/commentcorrection/21289170-19330002, http://linkedlifedata.com/resource/pubmed/commentcorrection/21289170-19357334, http://linkedlifedata.com/resource/pubmed/commentcorrection/21289170-19424431, http://linkedlifedata.com/resource/pubmed/commentcorrection/21289170-19515924, http://linkedlifedata.com/resource/pubmed/commentcorrection/21289170-19584315, http://linkedlifedata.com/resource/pubmed/commentcorrection/21289170-19641114, http://linkedlifedata.com/resource/pubmed/commentcorrection/21289170-19675236, http://linkedlifedata.com/resource/pubmed/commentcorrection/21289170-19872732, http://linkedlifedata.com/resource/pubmed/commentcorrection/21289170-19917569, http://linkedlifedata.com/resource/pubmed/commentcorrection/21289170-20062532, http://linkedlifedata.com/resource/pubmed/commentcorrection/21289170-20107081, http://linkedlifedata.com/resource/pubmed/commentcorrection/21289170-20220080, http://linkedlifedata.com/resource/pubmed/commentcorrection/21289170-2154487, http://linkedlifedata.com/resource/pubmed/commentcorrection/21289170-228196, http://linkedlifedata.com/resource/pubmed/commentcorrection/21289170-2324020, http://linkedlifedata.com/resource/pubmed/commentcorrection/21289170-2458455, http://linkedlifedata.com/resource/pubmed/commentcorrection/21289170-2461723, http://linkedlifedata.com/resource/pubmed/commentcorrection/21289170-2468634, http://linkedlifedata.com/resource/pubmed/commentcorrection/21289170-2473197, http://linkedlifedata.com/resource/pubmed/commentcorrection/21289170-2600628, http://linkedlifedata.com/resource/pubmed/commentcorrection/21289170-3656183, http://linkedlifedata.com/resource/pubmed/commentcorrection/21289170-4812270, http://linkedlifedata.com/resource/pubmed/commentcorrection/21289170-5810617, http://linkedlifedata.com/resource/pubmed/commentcorrection/21289170-6601693, http://linkedlifedata.com/resource/pubmed/commentcorrection/21289170-7543944, http://linkedlifedata.com/resource/pubmed/commentcorrection/21289170-8377824, http://linkedlifedata.com/resource/pubmed/commentcorrection/21289170-8804626, http://linkedlifedata.com/resource/pubmed/commentcorrection/21289170-8887774, http://linkedlifedata.com/resource/pubmed/commentcorrection/21289170-8986835, http://linkedlifedata.com/resource/pubmed/commentcorrection/21289170-9120577, http://linkedlifedata.com/resource/pubmed/commentcorrection/21289170-9491988, http://linkedlifedata.com/resource/pubmed/commentcorrection/21289170-957159
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1529-2401
More...