Source:http://linkedlifedata.com/resource/pubmed/id/21288137
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
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| pubmed:dateCreated |
2011-5-26
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| pubmed:abstractText |
Of forty-seven extended-spectrum cephalosporin-resistant Escherichia coli isolates, collected from children at the Children's Hospital in 2006 (Tunis, Tunisia), we analyzed 32 isolates that were genotypically different by enterobacterial repetitive intergenic consensus -polymerase chain reaction. For all isolates, the double-disk diffusion test revealed synergy between clavulanate and cefotaxime and/or ceftazidime, suggesting the production of extended-spectrum beta-lactamases. Polymerase chain reaction experiments, performed on plasmid DNA, and sequencing revealed the presence of bla(TEM-1B) (26 isolates, 81%), bla(TEM-34(IRT-6)) (3 isolates, 9%), bla(SHV-12) (2 isolates, 6%), and bla(CTX-M-15) (31 isolates, 97%). Further, the insertion sequence ISEcp1 was found upstream from the bla(CTX-M-15) gene in 11 isolates. The bla genes were found alone or in various combinations in a single isolate. bla(TEM-1B) and bla(CTX-M-15) genes were detected in 26 out of the 32 isolates. Three isolates harbored both bla(TEM-34(IRT-6)) and bla(CTX-M-15). bla(SHV-12) was identified either alone or with bla(CTX-M-15) in a single isolate. Our investigation showed the dominance of CTX-M-type extended-spectrum beta-lactamases, with CTX-M-15 particularly common, and to our best knowledge, this is the first report of the coexistence of CTX-M-15 and IRT-6 in E. coli isolates from children in Tunisia.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Anti-Bacterial Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Cefotaxime,
http://linkedlifedata.com/resource/pubmed/chemical/Ceftazidime,
http://linkedlifedata.com/resource/pubmed/chemical/Clavulanic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Bacterial,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Intergenic,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Transposable Elements,
http://linkedlifedata.com/resource/pubmed/chemical/beta-Lactamases,
http://linkedlifedata.com/resource/pubmed/chemical/beta-lactamase CTX-M-15
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| pubmed:status |
MEDLINE
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| pubmed:month |
Jun
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| pubmed:issn |
1931-8448
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| pubmed:author | |
| pubmed:issnType |
Electronic
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| pubmed:volume |
17
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
135-40
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| pubmed:meshHeading |
pubmed-meshheading:21288137-Anti-Bacterial Agents,
pubmed-meshheading:21288137-Bacterial Typing Techniques,
pubmed-meshheading:21288137-Cefotaxime,
pubmed-meshheading:21288137-Ceftazidime,
pubmed-meshheading:21288137-Child,
pubmed-meshheading:21288137-Clavulanic Acid,
pubmed-meshheading:21288137-DNA, Bacterial,
pubmed-meshheading:21288137-DNA, Intergenic,
pubmed-meshheading:21288137-DNA Transposable Elements,
pubmed-meshheading:21288137-Disk Diffusion Antimicrobial Tests,
pubmed-meshheading:21288137-Drug Resistance, Bacterial,
pubmed-meshheading:21288137-Escherichia coli,
pubmed-meshheading:21288137-Escherichia coli Infections,
pubmed-meshheading:21288137-Feces,
pubmed-meshheading:21288137-Humans,
pubmed-meshheading:21288137-Plasmids,
pubmed-meshheading:21288137-Polymerase Chain Reaction,
pubmed-meshheading:21288137-Tunisia,
pubmed-meshheading:21288137-beta-Lactamases
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| pubmed:year |
2011
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| pubmed:articleTitle |
Emergence and dominance of CTX-M-15 extended spectrum beta-lactamase among Escherichia coli isolates from children.
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| pubmed:affiliation |
Département des Sciences Biologiques, Faculté des Sciences de Tunis, Université Tunis El-Manar, Tunis, Tunisia. samia_biochimie@yahoo.fr
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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