Linked Life Data

21285868

Source:http://linkedlifedata.com/resource/pubmed/id/21285868

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2011-6-9
pubmed:abstractText
In biliary atresia (BA), a cholangiopathy of elusive etiology invariably leads to cirrhosis, and a disturbed angiogenesis may be involved. We evaluated the hepatobiliary immunolocalization of vascular endothelial growth factor (VEGF) A, VEGF receptor 1 (R1), and R2 in BA. We analyzed biopsies obtained at portoenterostomy from infants with BA (n=52), including embryonic (n=14) and perinatal (n=38) types. Controls were infants with intrahepatic cholestasis (IC; n=7). In BA, VEGF A immunolocalization was also evaluated in explants (n=33) and at the porta hepatis (n=16). We morphometrically assessed the percentage of CK7 (PCK7) positivity in BA and the ratio medial layer thickness/luminal diameter in hepatic artery branches in BA and IC. We found that arteries were more frequently positive for VEGF A in BA at portoenterostomy (P=0.006) than in other groups. In explants, VEGF A immunolocalization was mainly lobular (P<0.001). VEGFR2 was less frequently positive in BA than IC in bile ducts (P=0.023) and hepatocytes (P=0.011). A higher PCK7 positivity was associated with arterial (P<0.001) and biliary (P=0.040) VEGF A positivity. PCK7 was correlated with biliary (P=0.031), arterial (P=0.031), and hepatocytic (P=0.032) VEGF A positivity in BA at portoenterostomy. VEGF A was positive in arteries and bile ducts at the porta hepatis mainly in the perinatal BA type (P=0.013). Biliary (P=0.016) and arterial (P=0.044) VEGF A positivity were associated with higher ratio medial layer thickness/luminal diameter values. Our findings suggest that hypoxia/ischemia affects the portal structures in BA at portoenterostomy, beginning at the porta hepatis, and it is associated both with the extent of biliary proliferation and medial layer thickening.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
1533-4058
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
19
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
360-8
pubmed:meshHeading
pubmed-meshheading:21285868-Biliary Atresia, pubmed-meshheading:21285868-Biopsy, pubmed-meshheading:21285868-Cholestasis, Intrahepatic, pubmed-meshheading:21285868-Female, pubmed-meshheading:21285868-Hepatic Artery, pubmed-meshheading:21285868-Humans, pubmed-meshheading:21285868-Immunohistochemistry, pubmed-meshheading:21285868-Infant, Newborn, pubmed-meshheading:21285868-Keratin-7, pubmed-meshheading:21285868-Liver, pubmed-meshheading:21285868-Male, pubmed-meshheading:21285868-Microscopy, pubmed-meshheading:21285868-Portoenterostomy, Hepatic, pubmed-meshheading:21285868-Tunica Media, pubmed-meshheading:21285868-Vascular Endothelial Growth Factor A, pubmed-meshheading:21285868-Vascular Endothelial Growth Factor Receptor-1, pubmed-meshheading:21285868-Vascular Endothelial Growth Factor Receptor-2
pubmed:year
2011
pubmed:articleTitle
Immunolocalization of VEGF A and its receptors, VEGFR1 and VEGFR2, in the liver from patients with biliary atresia.
pubmed:affiliation
Laboratório Experimental de Hepatologia e Gastrenterologia do Centro de Pesquisas do Hospital de Clínicas de Porto Alegre, Porto Alegre, Rio Grande do Sul, Brazil.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't