Linked Life Data

2128364

Source:http://linkedlifedata.com/resource/pubmed/id/2128364

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1991-5-23
pubmed:abstractText
The authors present a simple and sensitive culture technique for isolation of Human Immunodeficiency Virus (HIV) from small amounts of heparinized whole blood. This method appeared to be highly sensitive at all stages of disease. Among asymptomatics HIV was isolated from 41.3% of subjects by using peripheral blood mononuclear cells in a standard co-cultivation technique and in 89.6% of cases using whole blood. In the group of AIDS patients, in contrast, HIV isolation rate of the two techniques was the same (94.4%). The presence of detectable p24 Ag in plasma did not affect cultural results and no false positive was observed in the control group. Although further studies are needed to better understand the biological significance of a positive cultural result obtained by this method, HIV isolation from whole blood can be routinely employed, especially when small amounts of blood are available.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0391-5352
pubmed:author
pubmed:issnType
Print
pubmed:volume
13
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
311-5
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
HIV isolation from whole blood: a new approach to HIV detection.
pubmed:affiliation
Clinic of Infectious Diseases, University of Bari, Policlinico Hospital, Italy.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't