Source:http://linkedlifedata.com/resource/pubmed/id/21282027
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
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| pubmed:dateCreated |
2011-2-25
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| pubmed:abstractText |
Ceramides and cerebrosides are key compounds in the metabolism of sphingolipids. Produced in response to a variety of apoptotic stimuli, these metabolites mediate either mitogenic or apoptotic responses, depending on cell type and nature of stimulus. Novel strategies using these selective targets for a therapeutic intervention, e.g. in cancer, cardiovascular and neurodegenerative diseases, and HIV, have been developed, along with anticancer approaches using controlled delivery of exogenous natural ceramides from ceramide-based liposomes. Thus, great is the need to find selective and sensitive analytical methods allowing a prompt detection of ceramides and cerebrosides in natural matrices. Here we report an analytical study carried out on the Amazonian plant Dracontium loretense, resulted in a preliminary analysis a rich source of this class of natural compounds. A handy, selective, and sensitive methodology based on high-performance liquid chromatography coupled to electrospray negative ionization multistage ion trap mass spectrometry (HPLC-ESI/ITMS(n)) was developed. Analysis of fingerprint multistage mass spectra allowed the rapid identification of 3 major long-chain bases and their exact pairing with 11 different fatty acids and with carbohydrate headgroups. Thus, the structures of 21 ceramide and cerebroside species, among which 7 molecules never reported before, were unambiguously assigned. Results obtained in this study demonstrated that this analytical approach could provide a reliable and sensitive method to obtain the qualitative on-line profiling of ceramides and cerebrosides in new medicinal plant matrices.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Apr
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| pubmed:issn |
1873-264X
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| pubmed:author | |
| pubmed:copyrightInfo |
Copyright © 2011 Elsevier B.V. All rights reserved.
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| pubmed:issnType |
Electronic
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| pubmed:day |
28
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| pubmed:volume |
55
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
23-30
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| pubmed:meshHeading |
pubmed-meshheading:21282027-Antineoplastic Agents, Phytogenic,
pubmed-meshheading:21282027-Araceae,
pubmed-meshheading:21282027-Ceramides,
pubmed-meshheading:21282027-Cerebrosides,
pubmed-meshheading:21282027-Chromatography, High Pressure Liquid,
pubmed-meshheading:21282027-Drug Discovery,
pubmed-meshheading:21282027-Magnetic Resonance Spectroscopy,
pubmed-meshheading:21282027-Medicine, Traditional,
pubmed-meshheading:21282027-Molecular Structure,
pubmed-meshheading:21282027-Plant Extracts,
pubmed-meshheading:21282027-Plant Roots,
pubmed-meshheading:21282027-South America,
pubmed-meshheading:21282027-Spectrometry, Mass, Electrospray Ionization,
pubmed-meshheading:21282027-Tandem Mass Spectrometry,
pubmed-meshheading:21282027-Technology, Pharmaceutical
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| pubmed:year |
2011
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| pubmed:articleTitle |
Qualitative on-line profiling of ceramides and cerebrosides by high performance liquid chromatography coupled with electrospray ionization ion trap tandem mass spectrometry: the case of Dracontium loretense.
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| pubmed:affiliation |
Dipartimento di Scienze Farmaceutiche, Università degli Studi di Salerno, Via Ponte Don Melillo, I-84084 Fisciano, SA, Italy.
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| pubmed:publicationType |
Journal Article
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