Linked Life Data

21279616

Source:http://linkedlifedata.com/resource/pubmed/id/21279616

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PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2011-1-31
pubmed:abstractText
Stem cell-based therapy has the potential to improve the prospect of patients suffering from many untreatable diseases. Applications of stem cells for therapy of neurological conditions, such as Parkinson's disease, Alzheimer's disease, multiple sclerosis, or stroke, are particularly appealing as damage of the central nervous system is irreversible and the efficacy of conventional therapy is limited. Despite a broad interest among researchers and clinicians, progress in this field has been slow due to the remarkable complexity of the brain, which makes the task of repairing damaged tissue with stem cells extremely challenging. Making advances can be expedited by novel technologies that can monitor transplanted cells non-invasively. In vivo cellular imaging allows for the repetitive, real-time observation of targeted cells over the course of treatment without the need for invasive biopsies. Magnetic resonance imaging (MRI) is an excellent non-invasive cellular imaging modality as it has superior resolution, is widely used clinically, and has no radiation. This technique currently requires magnetic labeling of cells using superparamagnetic iron oxide particles (SPIOs) and transfection agents. In this chapter, methods for cellular labeling with SPIOs, transplantation of stem cells into the mouse brain, and MR imaging of the cells both in vivo and postmortem will be described. Additional histological and immunohistochemical procedures for analysis of the transplanted cells and the diseased brain environment are also provided.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1940-6029
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
711
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
435-49
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
MRI of transplanted neural stem cells.
pubmed:affiliation
Russell H. Morgan Department of Radiology and Radiological Science, Division of MR Research, and Cellular Imaging Section, Institute for Cell Engineering, The Johns Hopkins University School of Medicine, Baltimore, MD, USA. staceycromer.berman@jhmi.edu
pubmed:publicationType
Journal Article