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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1991-5-1
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pubmed:abstractText |
Phenylalanine, tyrosine and dihydroxyphenylalanine (DOPA) were identified unambiguously and quantitatively determined in single ventral thoracic nerve cords from the locust, Schistocerca gregaria, by gas chromatography-negative-ion chemical ionisation mass spectrometry. Deuterium-labelled analogues of each compound were added to a single ventral thoracic nerve cord in hydrochloric acid; the tissue was homogenised and the suspension centrifuged. The remaining hydrochloric acid was eliminated azeotropically by repeated additions of acetonitrile followed by evaporation under a stream of nitrogen and the resultant residue derivatised by reaction with hexafluoroisopropanol and pentafluoropropionic anhydride. Under negative-ion chemical ionisation conditions, the hexafluoroisopropanol-pentafluoropropionyl derivatives produced characteristic ions which were sufficiently abundant to be suitable for selected-ion monitoring. This method is highly specific and gave a limit of detection below the nanogram level. The amounts of phenylalanine, tyrosine and DOPA in a single ventral thoracic nerve cord were, respectively, 194 +/- 81, 347 +/- 88 and 11 +/- 11 ng per tissue.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Deuterium,
http://linkedlifedata.com/resource/pubmed/chemical/Dihydroxyphenylalanine,
http://linkedlifedata.com/resource/pubmed/chemical/Fluorocarbons,
http://linkedlifedata.com/resource/pubmed/chemical/Phenylalanine,
http://linkedlifedata.com/resource/pubmed/chemical/Propanols,
http://linkedlifedata.com/resource/pubmed/chemical/Tyrosine,
http://linkedlifedata.com/resource/pubmed/chemical/hexafluoroisopropanol
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0021-9673
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
26
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pubmed:volume |
532
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1-11
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:2127782-Animals,
pubmed-meshheading:2127782-Deuterium,
pubmed-meshheading:2127782-Dihydroxyphenylalanine,
pubmed-meshheading:2127782-Female,
pubmed-meshheading:2127782-Fluorocarbons,
pubmed-meshheading:2127782-Gas Chromatography-Mass Spectrometry,
pubmed-meshheading:2127782-Grasshoppers,
pubmed-meshheading:2127782-Male,
pubmed-meshheading:2127782-Nervous System,
pubmed-meshheading:2127782-Phenylalanine,
pubmed-meshheading:2127782-Propanols,
pubmed-meshheading:2127782-Thorax,
pubmed-meshheading:2127782-Tyrosine
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pubmed:year |
1990
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pubmed:articleTitle |
Identification and quantitation of phenylalanine, tyrosine and dihydroxyphenylalanine in the thoracic nervous system of the locust, Schistocerca gregaria, by gas chromatography-negative-ion chemical ionisation mass spectrometry.
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pubmed:affiliation |
Department of Pharmacy, University of Strathclyde, Glasgow, Scotland, U.K.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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