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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
1991-5-1
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pubmed:abstractText |
Interferons (IFNs) induce the expression of a variety of cellular RNAs. Phorbol esters can inhibit IFN-induced expression of some of these RNAs, including ISG-54K. The actions of phorbol esters on IFN-activated ISG-54K transcription are cell specific and are reversed by inhibitors of protein synthesis. In those cell lines in which phorbol esters inhibit IFN-induced ISG-54K transcription, prolonged IFN exposure also induces a "desensitized state" such that further IFN exposure no longer induces ISG-54K expression. IFN-induced desensitization is also reversed by inhibitors of protein synthesis. Experiments are described to determine whether the mechanism by which phorbol esters inhibit IFN-activated ISG-54K expression is the same as the mechanism by which prolonged exposure to IFN makes cells refractory to further induction of ISG-54K expression. Cultured cells treated with 12-O-tetradecanoylphorbol 13-acetate (TPA) for 72 h are desensitized to phorbol esters such that further addition of phorbols does not inhibit IFN-induced ISG-54K expression. In both naive and TPA-desensitized human fibroblasts or WISH cells, prolonged IFN treatment induced a desensitized state that was reversible by cycloheximide. This observation suggests that the mechanisms by which prolonged IFN treatment and phorbol esters inhibit ISG-54K expression are independent.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2127700-2429366,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2127700-2441659,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2127700-2464596,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2127700-2474543,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2127700-2494657,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2127700-2538838,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2127700-2572326,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2127700-2821407,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2127700-2934388,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2127700-2945205,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2127700-3000601,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2127700-3037355,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2127700-3119989,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2127700-3134657,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2127700-3371658,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2127700-3455773,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2127700-3466167,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2127700-3476954,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2127700-3491822,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2127700-3540941,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2127700-3925348,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2127700-518835,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2127700-6436820,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2127700-6548307,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2127700-6548414
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
1044-2030
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
1
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pubmed:geneSymbol |
ISG-54K
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
707-13
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:2127700-Cells, Cultured,
pubmed-meshheading:2127700-Gene Expression Regulation,
pubmed-meshheading:2127700-Humans,
pubmed-meshheading:2127700-Interferon-gamma,
pubmed-meshheading:2127700-Kinetics,
pubmed-meshheading:2127700-Phorbol Esters,
pubmed-meshheading:2127700-Protein Synthesis Inhibitors,
pubmed-meshheading:2127700-RNA, Antisense,
pubmed-meshheading:2127700-RNA Probes,
pubmed-meshheading:2127700-Recombinant Proteins,
pubmed-meshheading:2127700-Transcription, Genetic
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pubmed:year |
1990
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pubmed:articleTitle |
Characterization of two independent mechanisms by which interferon-induced gene expression is down regulated.
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pubmed:affiliation |
Laboratory of Cytokine Research, Center for Biologics Evaluation and Research, National Institutes of Health, Bethesda, Maryland 20892.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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