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rdf:type |
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lifeskim:mentions |
umls-concept:C0003483,
umls-concept:C0004927,
umls-concept:C0017262,
umls-concept:C0021469,
umls-concept:C0038185,
umls-concept:C0086418,
umls-concept:C0185117,
umls-concept:C0205263,
umls-concept:C0600210,
umls-concept:C0998656,
umls-concept:C1135918,
umls-concept:C1150183,
umls-concept:C1264633,
umls-concept:C1280500,
umls-concept:C1556095,
umls-concept:C2911684
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pubmed:issue |
4
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pubmed:dateCreated |
2011-5-3
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pubmed:abstractText |
Atherosclerosis is accompanied by the proliferation of human aortic smooth muscle cells (HASMC) and their movement into the intima. Many reports have indicated the involvement of gelatinases (MMP-9 and MMP-2) in this pathogenesis. The ethylacetate fraction from starfish, Asterias amurensis (EFA), harvested from the Korean seaside has an inhibitory effect on MMP-9 and MMP-2 activities, as well as on the expression of MMP-9 in TNF-? induced HASMC in a dose-dependent manner. Also, EFA inhibits the migration of TNF-? induced HASMC in transwells containing gelatin coated plugs. EFA was not cytotoxic to HASMC over the range 0-1mg/ml. By Western-blot analysis, it was revealed that the phosphorylation of extracellular signal regulated kinase (ERK) in TNF-? induced cells was inhibited and nuclear factor kappa B (NF-?B) p65 levels in nuclear extracts were decreased by EFA treatment. In addition, ERK inhibitor (U0126) treated cells exhibited decreased MMP-9 activity in the zymographic assay. From these results, it was found that the gelatinolytic activity was regulated (1) by enzymatic inhibition of both MMP-9 and MMP-2, as well as (2) by the decreased production of MMP-9 via ERK pathways in EFA treated HASMCs. Taken together, it has been shown that EFA has a putative anti-atherosclerotic effect.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
1879-3177
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pubmed:author |
pubmed-author:ChangHyeun-WookHW,
pubmed-author:ChangYoung-ChaeYC,
pubmed-author:KimCheorl-HoCH,
pubmed-author:KimDong-SooDS,
pubmed-author:KimJeong-RanJR,
pubmed-author:KoHyun-KwonHK,
pubmed-author:KwonKyung-MinKM,
pubmed-author:LeeYoung-ChoonYC,
pubmed-author:NaMin-KyunMK,
pubmed-author:ParkSung-JaeSJ,
pubmed-author:SonJong-KeunJK,
pubmed-author:SongKwon-HoKH,
pubmed-author:SuhSeok-JongSJ,
pubmed-author:YangJu HyeJH
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pubmed:copyrightInfo |
Copyright © 2011 Elsevier Ltd. All rights reserved.
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pubmed:issnType |
Electronic
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pubmed:volume |
25
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
767-73
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pubmed:meshHeading |
pubmed-meshheading:21276846-Acetates,
pubmed-meshheading:21276846-Animals,
pubmed-meshheading:21276846-Aorta,
pubmed-meshheading:21276846-Asterias,
pubmed-meshheading:21276846-Blotting, Western,
pubmed-meshheading:21276846-Cell Movement,
pubmed-meshheading:21276846-Dose-Response Relationship, Drug,
pubmed-meshheading:21276846-Extracellular Signal-Regulated MAP Kinases,
pubmed-meshheading:21276846-Humans,
pubmed-meshheading:21276846-Korea,
pubmed-meshheading:21276846-Matrix Metalloproteinase 2,
pubmed-meshheading:21276846-Matrix Metalloproteinase 9,
pubmed-meshheading:21276846-Myocytes, Smooth Muscle,
pubmed-meshheading:21276846-Transcription Factor RelA,
pubmed-meshheading:21276846-Tumor Necrosis Factor-alpha
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pubmed:year |
2011
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pubmed:articleTitle |
Ethylacetate fraction from Korean seaside starfish, Asterias amurensis, has an inhibitory effect on MMP-9 activity and expression and on migration behavior of TNF-? induced human aortic smooth muscle cells.
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pubmed:affiliation |
College of Pharmacy, Yeungnam University, Gyeongsan 712-749, Republic of Korea.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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