Linked Life Data

2127679

Source:http://linkedlifedata.com/resource/pubmed/id/2127679

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1991-4-29
pubmed:abstractText
A clinical therapy for psoriasis, a hyperproliferative disease of the skin, utilizes topical application of crude coal tar sometimes followed by UV irradiation (Goeckerman therapy). To investigate the formation of covalent DNA adducts resulting from this therapy, skin biopsies were obtained from treated patients and controls. Indirect immunofluorescence staining with antisera generated against benzo(a)pyrene diol epoxide-modified DNA was used to investigate cell-specific localization of adduct formation. Specific nuclear staining was detected in the epidermal cells of all biopsies from treated patients but not from control biopsies obtained from untreated individuals. 32P postlabeling of DNA isolated from the biopsies was used to determine the spectrum of hydrophobic adducts present. A pattern of multiple adducts was detected in the samples obtained from the treated patients but not from controls.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1011-0283
pubmed:author
pubmed:issnType
Print
pubmed:volume
3
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
171-9
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
Detection of DNA adducts in skin biopsies of coal tar-treated psoriasis patients: immunofluorescence and 32P postlabeling.
pubmed:affiliation
Cancer Center, School of Public Health, Columbia University, New York, N.Y.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't