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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
10
|
pubmed:dateCreated |
1991-4-23
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pubmed:databankReference | |
pubmed:abstractText |
The minimal region encoding the Corynebacterium glutamicum threonine synthase structural gene and its promoter was mapped by deletion analysis and complementation of the C. glutamicum thrC allele to a 1.6 kb region of the recombinant plasmid pFS80. The nucleotide sequence of this and flanking DNA was determined. The transcription and translation start points were identified by S1 mapping analysis and amino-terminal protein sequencing, respectively. The thrC gene encodes a 54481-Dalton polypeptide product. Translation of the thrC mRNA initiates only six nucleotides downstream from transcription. The length of the mRNA transcript is consistent with a single gene transcription unit. The C. glutamicum thrC gene is expressed independently of the other threonine-specific genes hom and thrB.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0950-382X
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
4
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pubmed:geneSymbol |
hom,
thrB,
thrC
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pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
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pubmed:pagination |
1693-702
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:2127631-Alleles,
pubmed-meshheading:2127631-Amino Acid Sequence,
pubmed-meshheading:2127631-Bacillus subtilis,
pubmed-meshheading:2127631-Blotting, Northern,
pubmed-meshheading:2127631-Carbon-Oxygen Lyases,
pubmed-meshheading:2127631-Corynebacterium,
pubmed-meshheading:2127631-Escherichia coli,
pubmed-meshheading:2127631-Genes, Bacterial,
pubmed-meshheading:2127631-Lyases,
pubmed-meshheading:2127631-Molecular Sequence Data,
pubmed-meshheading:2127631-Molecular Structure,
pubmed-meshheading:2127631-Plasmids,
pubmed-meshheading:2127631-Protein Biosynthesis,
pubmed-meshheading:2127631-RNA, Messenger,
pubmed-meshheading:2127631-Sequence Homology, Nucleic Acid,
pubmed-meshheading:2127631-Transcription, Genetic
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pubmed:year |
1990
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pubmed:articleTitle |
The molecular structure of the Corynebacterium glutamicum threonine synthase gene.
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pubmed:affiliation |
Seoul Miwon Co. Ltd, Dobong-ku, Korea.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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