Linked Life Data

21276152

Source:http://linkedlifedata.com/resource/pubmed/id/21276152

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2011-2-22
pubmed:abstractText
Aim:? Studies of the complete hepatitis C virus (HCV) life cycle have become possible with the development of a HCV-JFH1 cell culture system. Methods:? In this study, we constructed two fluorescence protein-tagged recombinant JFH1 virus clones, JFH1-EYFP and JFH1-AsRed, as well as two corresponding clones with adaptive mutations, JFH1-EYFP mutant and JFH1-AsRed mutant, that and were as effective as JFH1 in producing infectious virus particles, and investigated their viral infection life cycles. Results:? After infection of the fluorescence-tagged mutant viruses, infected cells increased exponentially. In cells, EYFP or AsRed and NS5A were expressed as a fusion protein and co-localized in core proteins. The rate of the cell-cell spread was dependent on the cell densities with a maximum of 10(2.5) /day. Treatment of cells with interferon or a protease inhibitor suppressed expansion of virus-positive cells. Conclusion:? Taken together, these results indicate that fluorescence-tagged HCV is a useful tool to study virus infection life cycles and to assist in the search for novel antiviral compounds.
pubmed:language
eng
pubmed:journal
pubmed:status
PubMed-not-MEDLINE
pubmed:month
Mar
pubmed:issn
1386-6346
pubmed:author
pubmed:copyrightInfo
© 2011 The Japan Society of Hepatology.
pubmed:issnType
Print
pubmed:volume
41
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
258-69
pubmed:year
2011
pubmed:articleTitle
Studies on virus kinetics using infectious fluorescence-tagged hepatitis C virus cell culture.
pubmed:affiliation
Department of Gastroenterology and Hepatology Department for Hepatitis Control Department of Advanced Therapeutics in Gastrointestinal Diseases, Tokyo Medical and Dental University Department of Virology II, National Institute of Infectious Disease, Tokyo Department of Internal Medicine, Soka Municipal Hospital, Saitama, Japan.
pubmed:publicationType
Journal Article