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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6021
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pubmed:dateCreated |
2011-3-9
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pubmed:databankReference |
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pubmed:abstractText |
Centrioles are cylindrical, ninefold symmetrical structures with peripheral triplet microtubules strictly required to template cilia and flagella. The highly conserved protein SAS-6 constitutes the center of the cartwheel assembly that scaffolds centrioles early in their biogenesis. We determined the x-ray structure of the amino-terminal domain of SAS-6 from zebrafish, and we show that recombinant SAS-6 self-associates in vitro into assemblies that resemble cartwheel centers. Point mutations are consistent with the notion that centriole formation in vivo depends on the interactions that define the self-assemblies observed here. Thus, these interactions are probably essential to the structural organization of cartwheel centers.
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pubmed:grant |
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
1095-9203
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pubmed:author |
pubmed-author:AndreevaAntoninaA,
pubmed-author:EbongIma-ObongIO,
pubmed-author:HironoMasafumiM,
pubmed-author:JohnsonChristopher MCM,
pubmed-author:MorgnerNinaN,
pubmed-author:NakazawaYukiY,
pubmed-author:PetrovichMirianaM,
pubmed-author:RobinsonCarol VCV,
pubmed-author:VeprintsevDmitryD,
pubmed-author:YamaguchiShokoS,
pubmed-author:YanagisawaHaru-akiHA,
pubmed-author:ZuberBenoîtB,
pubmed-author:van BreugelMarkM
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pubmed:issnType |
Electronic
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pubmed:day |
4
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pubmed:volume |
331
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1196-9
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pubmed:meshHeading |
pubmed-meshheading:21273447-Amino Acid Motifs,
pubmed-meshheading:21273447-Animals,
pubmed-meshheading:21273447-Cell Cycle Proteins,
pubmed-meshheading:21273447-Cell Line, Tumor,
pubmed-meshheading:21273447-Centrioles,
pubmed-meshheading:21273447-Centrosome,
pubmed-meshheading:21273447-Chlamydomonas reinhardtii,
pubmed-meshheading:21273447-Chromosomal Proteins, Non-Histone,
pubmed-meshheading:21273447-Crystallography, X-Ray,
pubmed-meshheading:21273447-Flagella,
pubmed-meshheading:21273447-Humans,
pubmed-meshheading:21273447-Hydrophobic and Hydrophilic Interactions,
pubmed-meshheading:21273447-Models, Molecular,
pubmed-meshheading:21273447-Mutant Proteins,
pubmed-meshheading:21273447-Point Mutation,
pubmed-meshheading:21273447-Protein Multimerization,
pubmed-meshheading:21273447-Protein Structure, Quaternary,
pubmed-meshheading:21273447-Protein Structure, Tertiary,
pubmed-meshheading:21273447-Recombinant Proteins,
pubmed-meshheading:21273447-Zebrafish,
pubmed-meshheading:21273447-Zebrafish Proteins
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pubmed:year |
2011
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pubmed:articleTitle |
Structures of SAS-6 suggest its organization in centrioles.
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pubmed:affiliation |
Medical Research Council-Laboratory of Molecular Biology (MRC-LMB), Hills Road, Cambridge, UK. vanbreug@mrc-lmb.cam.ac.uk
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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