Linked Life Data

21272943

Source:http://linkedlifedata.com/resource/pubmed/id/21272943

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3-4
pubmed:dateCreated
2011-3-7
pubmed:databankReference
pubmed:abstractText
FOXP3 is a transcription factor predominantly expressed in CD4(+) CD25(+) thymocytes and CD4(+) CD25(+) peripheral T cells. It has been reported that variation in the FOXP3 gene (FOXP3) could cause impaired immune regulation. Using PCR-SSCP, variation in exon 4, exon 9-10 and exon 13-14 of ovine FOXP3, covering three functional domains (a proline rich domain, a leucine zipper-like motif and the forkhead domain, respectively) was investigated. Four SSCP banding patterns were observed for the exon 13-14 amplicon, while no variation was detected in either exon 4 or exon 9-10. Either one or two SSCP banding patterns were observed in the sheep studied. In accordance with the X chromosome location of FOXP3, ewes were homozygous or heterozygous, while rams were hemizygous. Sequencing of DNA corresponding to the four PCR-SSCP patterns for exon 13-14, revealed four unique DNA sequences which resulted from both sequence and length variation. These comprised of two SNPs (one in intron 13 and the other in exon 14), and variation in copy number for a hexa-nucleotide repeat (TGGCCC)(n) in intron 13. These results indicate that ovine FOXP3 is polymorphic and the variation detected in this region may have implications for immune regulation in sheep.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1873-2534
pubmed:author
pubmed:copyrightInfo
Copyright © 2011 Elsevier B.V. All rights reserved.
pubmed:issnType
Electronic
pubmed:day
15
pubmed:volume
140
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
303-6
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
Polymorphism of the ovine FOXP3 gene (FOXP3).
pubmed:affiliation
Gene-Marker Laboratory, Faculty of Agricultural and Life Sciences, PO Box 84, Lincoln University, Lincoln, New Zealand.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't