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pubmed-article:21269885rdf:typepubmed:Citationlld:pubmed
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pubmed-article:21269885pubmed:dateCreated2011-2-14lld:pubmed
pubmed-article:21269885pubmed:abstractTextThe aim of the present study was to compare an 'open' vitrification protocol to a 'closed' vitrification protocol for mature human oocytes. A prospective comparison between fresh and sibling vitrified oocytes and a retrospective comparison between the two vitrification protocols were performed. For recruited patients undergoing an IVF cycle, two or three fresh oocytes were inseminated with intracytoplasmic sperm injection (ICSI) and the remaining three or more oocytes were vitrified according to manufacturer's instructions with a 'closed' or an 'open' vitrification system. After an unsuccessful fresh cycle, oocytes were warmed and inseminated with ICSI. Embryological parameters were recorded and compared between fresh and sibling vitrified oocytes (intrapatient) as well as between the two vitrification techniques (interpatient). Oocytes vitrified with the 'closed' system showed significantly lower fertilization and cleavage rates and a reduction in the quantity and quality of obtained embryos compared with fresh sibling oocytes (P<0.001). On the contrary, the same parameters were similar between fresh and sibling oocytes vitrified using the 'open' system. The retrospective comparison between the two vitrification protocols also showed a significant increase in clinical pregnancy rate and a reduced proportion of cancelled cycles using the 'open' system (P<0.01).lld:pubmed
pubmed-article:21269885pubmed:languageenglld:pubmed
pubmed-article:21269885pubmed:journalhttp://linkedlifedata.com/r...lld:pubmed
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pubmed-article:21269885pubmed:issn1472-6491lld:pubmed
pubmed-article:21269885pubmed:authorpubmed-author:NicolosiAnna...lld:pubmed
pubmed-article:21269885pubmed:authorpubmed-author:RagniGuidoGlld:pubmed
pubmed-article:21269885pubmed:authorpubmed-author:RestelliLilia...lld:pubmed
pubmed-article:21269885pubmed:authorpubmed-author:PaffoniAlessi...lld:pubmed
pubmed-article:21269885pubmed:authorpubmed-author:FerrariStefan...lld:pubmed
pubmed-article:21269885pubmed:authorpubmed-author:ScarduelliCla...lld:pubmed
pubmed-article:21269885pubmed:authorpubmed-author:GuarneriCrist...lld:pubmed
pubmed-article:21269885pubmed:copyrightInfoCopyright © 2010 Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.lld:pubmed
pubmed-article:21269885pubmed:issnTypeElectroniclld:pubmed
pubmed-article:21269885pubmed:volume22lld:pubmed
pubmed-article:21269885pubmed:ownerNLMlld:pubmed
pubmed-article:21269885pubmed:authorsCompleteYlld:pubmed
pubmed-article:21269885pubmed:pagination292-8lld:pubmed
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pubmed-article:21269885pubmed:year2011lld:pubmed
pubmed-article:21269885pubmed:articleTitleEffects of two vitrification protocols on the developmental potential of human mature oocytes.lld:pubmed
pubmed-article:21269885pubmed:affiliationFondazione Ca' Granda Ospedale Maggiore Policlinico, Infertility Unit, Via M. Fanti 6, 20122 Milan, Italy. alessio.paffoni@alice.itlld:pubmed
pubmed-article:21269885pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:21269885pubmed:publicationTypeComparative Studylld:pubmed
pubmed-article:21269885pubmed:publicationTypeEvaluation Studieslld:pubmed