Linked Life Data

21266196

Source:http://linkedlifedata.com/resource/pubmed/id/21266196

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2011-2-28
pubmed:abstractText
SNXs (sorting nexin), a family of proteins playing roles in cargo sorting and signaling from compartments within the endocytic network, regulate traffic of membrane proteins including TGF-? receptors. Here we report that the full length human and mouse SNX25, a SNX member with PX, PXA and RGS domains, co-localizes with TGF-? receptors, and forms internalized cytosolic punctae upon treatment with TGF-?. While overexpression of SNX25 inhibits TGF-? induced luciferase reporter activity, knocking down endogenous SNX25 by siRNA in NIH3T3 cells elevates the TGF-? receptor levels and facilitates TGF-? signaling. Immunoprecipitation experiments demonstrate that SNX25 interacts with T?RI. Western blot analyses indicate that SNX25 enhances the degradation of TGF-? receptors. SNX25 induced TGF-? receptor degradation is shown via the clathrin dependent endocytosis pathway into lysosome. We have characterized that PXA domain of SNX25 is required for the degradation of T?RI. Our findings demonstrate that SNX25 negatively regulates TGF-? signaling by enhancing the receptor degradation through lysosome pathway.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1873-3913
pubmed:author
pubmed:copyrightInfo
Copyright © 2011 Elsevier Inc. All rights reserved.
pubmed:issnType
Electronic
pubmed:volume
23
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
935-46
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
SNX25 regulates TGF-? signaling by enhancing the receptor degradation.
pubmed:affiliation
Department of Hematology/Oncology, Affiliated Hospital, Hainan Medical College, Hainan (570102), China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't