Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1991-3-20
pubmed:abstractText
The regulation of the mitotic histone H1 kinase activity has been analyzed during the naturally synchronous cell cycle of Physarum polycephalum plasmodia. The universal binding property of the p13suc1 Schizosaccharomyces pombe gene product was used to precipitate and assay the cdc2 histone H1 kinase activity. The kinase activity peaks at the beginning of metaphase and its decline, which requires protein synthesis, appears to be an early event during the metaphase process. Microtubular poisons, temperature shifts and DNA synthesis inhibitors were used to perturb cell cycle regulatory pathways and characterize their effects on cdc2 kinase activation. Our results suggest that the full activation of the mitotic kinase requires at least two successive triggering signals involving microtubular components and DNA synthesis.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Aphidicolin, http://linkedlifedata.com/resource/pubmed/chemical/Benzimidazoles, http://linkedlifedata.com/resource/pubmed/chemical/CDC2 Protein Kinase, http://linkedlifedata.com/resource/pubmed/chemical/Carbamates, http://linkedlifedata.com/resource/pubmed/chemical/Cell Cycle Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Cycloheximide, http://linkedlifedata.com/resource/pubmed/chemical/DNA, Fungal, http://linkedlifedata.com/resource/pubmed/chemical/DNA-Directed DNA Polymerase, http://linkedlifedata.com/resource/pubmed/chemical/Diterpenes, http://linkedlifedata.com/resource/pubmed/chemical/Fungal Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Griseofulvin, http://linkedlifedata.com/resource/pubmed/chemical/Schizosaccharomyces pombe Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Suc1 protein, S pombe, http://linkedlifedata.com/resource/pubmed/chemical/mecarzole
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0021-9533
pubmed:author
pubmed:issnType
Print
pubmed:volume
96 ( Pt 4)
pubmed:geneSymbol
p13
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
683-9
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:2126544-Aphidicolin, pubmed-meshheading:2126544-Benzimidazoles, pubmed-meshheading:2126544-CDC2 Protein Kinase, pubmed-meshheading:2126544-Carbamates, pubmed-meshheading:2126544-Cell Cycle, pubmed-meshheading:2126544-Cell Cycle Proteins, pubmed-meshheading:2126544-Chromatography, Affinity, pubmed-meshheading:2126544-Cycloheximide, pubmed-meshheading:2126544-DNA, Fungal, pubmed-meshheading:2126544-DNA-Directed DNA Polymerase, pubmed-meshheading:2126544-Diterpenes, pubmed-meshheading:2126544-Fungal Proteins, pubmed-meshheading:2126544-Griseofulvin, pubmed-meshheading:2126544-Microtubules, pubmed-meshheading:2126544-Phosphorylation, pubmed-meshheading:2126544-Physarum, pubmed-meshheading:2126544-Schizosaccharomyces pombe Proteins, pubmed-meshheading:2126544-Signal Transduction
pubmed:year
1990
pubmed:articleTitle
Cell cycle regulation of p34cdc2 kinase activity in Physarum polycephalum.
pubmed:affiliation
Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, NY 11724.
pubmed:publicationType
Journal Article, In Vitro, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't