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rdf:type |
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lifeskim:mentions |
umls-concept:C0004112,
umls-concept:C0035647,
umls-concept:C0205224,
umls-concept:C0205374,
umls-concept:C0292777,
umls-concept:C0439855,
umls-concept:C0597357,
umls-concept:C1412494,
umls-concept:C1425291,
umls-concept:C1519952,
umls-concept:C2587213
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pubmed:issue |
6
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pubmed:dateCreated |
2011-2-9
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pubmed:abstractText |
Regulatory volume decrease (RVD) is a key mechanism for volume control that serves to prevent detrimental swelling in response to hypo-osmotic stress. The molecular basis of RVD is not understood. Here we show that a complex containing aquaporin-4 (AQP4) and transient receptor potential vanilloid 4 (TRPV4) is essential for RVD in astrocytes. Astrocytes from AQP4-KO mice and astrocytes treated with TRPV4 siRNA fail to respond to hypotonic stress by increased intracellular Ca(2+) and RVD. Coimmunoprecipitation and immunohistochemistry analyses show that AQP4 and TRPV4 interact and colocalize. Functional analysis of an astrocyte-derived cell line expressing TRPV4 but not AQP4 shows that RVD and intracellular Ca(2+) response can be reconstituted by transfection with AQP4 but not with aquaporin-1. Our data indicate that astrocytes contain a TRPV4/AQP4 complex that constitutes a key element in the brain's volume homeostasis by acting as an osmosensor that couples osmotic stress to downstream signaling cascades.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/21262839-10516112,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21262839-10960499,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21262839-11081638,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21262839-12765692,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21262839-1378628,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21262839-14682361,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21262839-14734638,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21262839-15489228,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21262839-15561405,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21262839-15561415,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21262839-15846797,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21262839-15923656,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21262839-16115206,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21262839-16293632,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21262839-16537379,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21262839-16571723,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21262839-16802708,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21262839-16805837,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21262839-16843084,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21262839-17064359,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21262839-17170210,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21262839-17215147,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21262839-17488344,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21262839-17693761,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21262839-17719182,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21262839-18355916,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21262839-18679106,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21262839-19027987,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21262839-19076362,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21262839-19705854,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21262839-8501529
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/AQP1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/AQP4 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Aqp1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Aqp4 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Aquaporin 1,
http://linkedlifedata.com/resource/pubmed/chemical/Aquaporin 4,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/TRPV Cation Channels,
http://linkedlifedata.com/resource/pubmed/chemical/TRPV4 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Trpv4 protein, mouse
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
1091-6490
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
8
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pubmed:volume |
108
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2563-8
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pubmed:dateRevised |
2011-8-25
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pubmed:meshHeading |
pubmed-meshheading:21262839-Animals,
pubmed-meshheading:21262839-Aquaporin 1,
pubmed-meshheading:21262839-Aquaporin 4,
pubmed-meshheading:21262839-Astrocytes,
pubmed-meshheading:21262839-COS Cells,
pubmed-meshheading:21262839-Calcium,
pubmed-meshheading:21262839-Cell Size,
pubmed-meshheading:21262839-Cercopithecus aethiops,
pubmed-meshheading:21262839-Cricetinae,
pubmed-meshheading:21262839-Humans,
pubmed-meshheading:21262839-Mice,
pubmed-meshheading:21262839-Mice, Knockout,
pubmed-meshheading:21262839-Osmotic Pressure,
pubmed-meshheading:21262839-Signal Transduction,
pubmed-meshheading:21262839-TRPV Cation Channels
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pubmed:year |
2011
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pubmed:articleTitle |
An aquaporin-4/transient receptor potential vanilloid 4 (AQP4/TRPV4) complex is essential for cell-volume control in astrocytes.
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pubmed:affiliation |
Institute for Nanostructured Materials, Consiglio Nazionale delle Ricerche, 40129 Bologna, Italy.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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