Linked Life Data

21261933

Source:http://linkedlifedata.com/resource/pubmed/id/21261933

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2011-1-25
pubmed:abstractText
Heterologous biosynthesis offers a new way to capture the medicinal properties presented by complex natural products. In this study, production of 6-deoxyerythronolide B (6dEB), the polyketide precursor to the antibiotic erythromycin, was used to probe the heterologous pathways needed for Escherichia coli-derived biosynthesis. More specifically, the heterologous proteins responsible for 6dEB production were varied by adjusting their respective gene dosage levels. In this way, heterologous components required for posttranslational modification, 6dEB biosynthesis, and substrate provision were adjusted in expression levels to observe the relative effect each has on final heterologous biosynthesis. The results indicate that both the biosynthetic and substrate provision heterologous proteins impact 6dEB formation to a greater extent when compared with posttranslational modification and suggest these components for future protein and metabolic engineering.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1751-7915
pubmed:author
pubmed:copyrightInfo
© 2009 The Authors; Journal compilation © 2009 Society for Applied Microbiology and Blackwell Publishing Ltd.
pubmed:issnType
Electronic
pubmed:volume
2
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
390-4
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Probing the heterologous metabolism supporting 6-deoxyerythronolide B biosynthesis in Escherichia coli.
pubmed:affiliation
Department of Chemical and Biological Engineering, Tufts University, Medford, MA 02155, USA.
pubmed:publicationType
Journal Article