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PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2011-2-28
pubmed:abstractText
In all eukaryotes, C/D small nucleolar ribonucleoproteins (C/D snoRNPs) are essential for methylation and processing of ribosomal RNAs. They consist of a box C/D small nucleolar RNA (C/D snoRNA) associated with four highly conserved nucleolar proteins. Recent data in HeLa cells and yeast have revealed that assembly of these snoRNPs is directed by NUFIP protein and other auxiliary factors. Nevertheless, the precise function and biological importance of NUFIP and the other assembly factors remains unknown. In plants, few studies have focused on RNA methylation and snoRNP biogenesis. Here, we identify and characterise the AtNUFIP gene that directs assembly of C/D snoRNP. To elucidate the function of AtNUFIP in planta, we characterized atnufip mutants. These mutants are viable but have severe developmental phenotypes. Northern blot analysis of snoRNA accumulation in atnufip mutants revealed a specific degradation of C/D snoRNAs and this situation is correlated with a reduction in rRNA methylation. Remarkably, the impact of AtNUFIP depends on the structure of snoRNA genes: it is essential for the accumulation of those C/D snoRNAs encoded by polycistronic genes, but not by monocistronic or tsnoRNA genes. We propose that AtNUFIP controls the kinetics of C/D snoRNP assembly on nascent precursors to overcome snoRNA degradation of aberrant RNPs. Finally, we show that AtNUFIP has broader RNP targets, controlling the accumulation of scaRNAs that direct methylation of spliceosomal snRNA in Cajal bodies.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
1365-313X
pubmed:author
pubmed:copyrightInfo
© 2011 The Authors. The Plant Journal © 2011 Blackwell Publishing Ltd.
pubmed:issnType
Electronic
pubmed:volume
65
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
807-19
pubmed:meshHeading
pubmed-meshheading:21261762-Amino Acid Sequence, pubmed-meshheading:21261762-Arabidopsis, pubmed-meshheading:21261762-Arabidopsis Proteins, pubmed-meshheading:21261762-Gene Expression Profiling, pubmed-meshheading:21261762-Genetic Complementation Test, pubmed-meshheading:21261762-Methylation, pubmed-meshheading:21261762-Molecular Sequence Data, pubmed-meshheading:21261762-Mutagenesis, Insertional, pubmed-meshheading:21261762-Mutation, pubmed-meshheading:21261762-Phenotype, pubmed-meshheading:21261762-Plants, Genetically Modified, pubmed-meshheading:21261762-RNA, Plant, pubmed-meshheading:21261762-RNA, Ribosomal, pubmed-meshheading:21261762-RNA, Small Nucleolar, pubmed-meshheading:21261762-RNA Processing, Post-Transcriptional, pubmed-meshheading:21261762-RNA Stability, pubmed-meshheading:21261762-Ribonucleoproteins, Small Nucleolar, pubmed-meshheading:21261762-Sequence Alignment
pubmed:year
2011
pubmed:articleTitle
AtNUFIP, an essential protein for plant development, reveals the impact of snoRNA gene organisation on the assembly of snoRNPs and rRNA methylation in Arabidopsis thaliana.
pubmed:affiliation
Laboratoire Génome et Développement des Plantes, UMR 5096 Université de Perpignan via Domitia - Centre National de la Recherche Scientifique - Institut de Recherche pour le Développement, Perpignan, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't