| pubmed-article:21256200 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:21256200 | lifeskim:mentions | umls-concept:C0014939 | lld:lifeskim |
| pubmed-article:21256200 | lifeskim:mentions | umls-concept:C1274040 | lld:lifeskim |
| pubmed-article:21256200 | lifeskim:mentions | umls-concept:C0023688 | lld:lifeskim |
| pubmed-article:21256200 | lifeskim:mentions | umls-concept:C1510438 | lld:lifeskim |
| pubmed-article:21256200 | lifeskim:mentions | umls-concept:C1705938 | lld:lifeskim |
| pubmed-article:21256200 | lifeskim:mentions | umls-concept:C1550605 | lld:lifeskim |
| pubmed-article:21256200 | lifeskim:mentions | umls-concept:C1704788 | lld:lifeskim |
| pubmed-article:21256200 | lifeskim:mentions | umls-concept:C0456205 | lld:lifeskim |
| pubmed-article:21256200 | lifeskim:mentions | umls-concept:C1527178 | lld:lifeskim |
| pubmed-article:21256200 | lifeskim:mentions | umls-concept:C0598002 | lld:lifeskim |
| pubmed-article:21256200 | pubmed:issue | 1-2 | lld:pubmed |
| pubmed-article:21256200 | pubmed:dateCreated | 2011-4-11 | lld:pubmed |
| pubmed-article:21256200 | pubmed:abstractText | Estrogen receptor-? (ER) is an important target both for therapeutic compounds and endocrine disrupting chemicals (EDCs); however, the mechanisms involved in chemical modulation of regulating ER transcriptional activity are inadequately understood. Here, we report the development of a high content analysis-based assay to describe ER activity that uniquely exploits a microscopically visible multi-copy integration of an ER-regulated promoter. Through automated single-cell analyses, we simultaneously quantified promoter occupancy, recruitment of transcriptional cofactors and large-scale chromatin changes in response to a panel of ER ligands and EDCs. Image-derived multi-parametric data was used to classify a panel of ligand responses at high resolution. We propose this system as a novel technology providing new mechanistic insights into EDC activities in a manner useful for both basic mechanistic studies and drug testing. | lld:pubmed |
| pubmed-article:21256200 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21256200 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21256200 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21256200 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21256200 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21256200 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21256200 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21256200 | pubmed:language | eng | lld:pubmed |
| pubmed-article:21256200 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21256200 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:21256200 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21256200 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21256200 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21256200 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21256200 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21256200 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21256200 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21256200 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21256200 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21256200 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21256200 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21256200 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:21256200 | pubmed:month | May | lld:pubmed |
| pubmed-article:21256200 | pubmed:issn | 1879-0038 | lld:pubmed |
| pubmed-article:21256200 | pubmed:author | pubmed-author:JonesE DED | lld:pubmed |
| pubmed-article:21256200 | pubmed:author | pubmed-author:ManciniM AMA | lld:pubmed |
| pubmed-article:21256200 | pubmed:author | pubmed-author:AshcroftF JFJ | lld:pubmed |
| pubmed-article:21256200 | pubmed:author | pubmed-author:NewbergJ YJY | lld:pubmed |
| pubmed-article:21256200 | pubmed:author | pubmed-author:MikicII | lld:pubmed |
| pubmed-article:21256200 | pubmed:copyrightInfo | Copyright © 2011 Elsevier B.V. All rights reserved. | lld:pubmed |
| pubmed-article:21256200 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:21256200 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:21256200 | pubmed:volume | 477 | lld:pubmed |
| pubmed-article:21256200 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:21256200 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:21256200 | pubmed:pagination | 42-52 | lld:pubmed |
| pubmed-article:21256200 | pubmed:meshHeading | pubmed-meshheading:21256200... | lld:pubmed |
| pubmed-article:21256200 | pubmed:meshHeading | pubmed-meshheading:21256200... | lld:pubmed |
| pubmed-article:21256200 | pubmed:meshHeading | pubmed-meshheading:21256200... | lld:pubmed |
| pubmed-article:21256200 | pubmed:meshHeading | pubmed-meshheading:21256200... | lld:pubmed |
| pubmed-article:21256200 | pubmed:meshHeading | pubmed-meshheading:21256200... | lld:pubmed |
| pubmed-article:21256200 | pubmed:meshHeading | pubmed-meshheading:21256200... | lld:pubmed |
| pubmed-article:21256200 | pubmed:meshHeading | pubmed-meshheading:21256200... | lld:pubmed |
| pubmed-article:21256200 | pubmed:meshHeading | pubmed-meshheading:21256200... | lld:pubmed |
| pubmed-article:21256200 | pubmed:meshHeading | pubmed-meshheading:21256200... | lld:pubmed |
| pubmed-article:21256200 | pubmed:meshHeading | pubmed-meshheading:21256200... | lld:pubmed |
| pubmed-article:21256200 | pubmed:meshHeading | pubmed-meshheading:21256200... | lld:pubmed |
| pubmed-article:21256200 | pubmed:meshHeading | pubmed-meshheading:21256200... | lld:pubmed |
| pubmed-article:21256200 | pubmed:meshHeading | pubmed-meshheading:21256200... | lld:pubmed |
| pubmed-article:21256200 | pubmed:meshHeading | pubmed-meshheading:21256200... | lld:pubmed |
| pubmed-article:21256200 | pubmed:meshHeading | pubmed-meshheading:21256200... | lld:pubmed |
| pubmed-article:21256200 | pubmed:meshHeading | pubmed-meshheading:21256200... | lld:pubmed |
| pubmed-article:21256200 | pubmed:meshHeading | pubmed-meshheading:21256200... | lld:pubmed |
| pubmed-article:21256200 | pubmed:year | 2011 | lld:pubmed |
| pubmed-article:21256200 | pubmed:articleTitle | High content imaging-based assay to classify estrogen receptor-? ligands based on defined mechanistic outcomes. | lld:pubmed |
| pubmed-article:21256200 | pubmed:affiliation | Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX 77030, USA. | lld:pubmed |
| pubmed-article:21256200 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:21256200 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| pubmed-article:21256200 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| pubmed-article:21256200 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
