Linked Life Data

21256200

Source:http://linkedlifedata.com/resource/pubmed/id/21256200

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
2011-4-11
pubmed:abstractText
Estrogen receptor-? (ER) is an important target both for therapeutic compounds and endocrine disrupting chemicals (EDCs); however, the mechanisms involved in chemical modulation of regulating ER transcriptional activity are inadequately understood. Here, we report the development of a high content analysis-based assay to describe ER activity that uniquely exploits a microscopically visible multi-copy integration of an ER-regulated promoter. Through automated single-cell analyses, we simultaneously quantified promoter occupancy, recruitment of transcriptional cofactors and large-scale chromatin changes in response to a panel of ER ligands and EDCs. Image-derived multi-parametric data was used to classify a panel of ligand responses at high resolution. We propose this system as a novel technology providing new mechanistic insights into EDC activities in a manner useful for both basic mechanistic studies and drug testing.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1879-0038
pubmed:author
pubmed:copyrightInfo
Copyright © 2011 Elsevier B.V. All rights reserved.
pubmed:issnType
Electronic
pubmed:day
15
pubmed:volume
477
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
42-52
pubmed:meshHeading
pubmed-meshheading:21256200-Blotting, Western, pubmed-meshheading:21256200-Cell Line, Tumor, pubmed-meshheading:21256200-Cluster Analysis, pubmed-meshheading:21256200-Endocrine Disruptors, pubmed-meshheading:21256200-Estradiol, pubmed-meshheading:21256200-Estrogen Antagonists, pubmed-meshheading:21256200-Estrogen Receptor alpha, pubmed-meshheading:21256200-Green Fluorescent Proteins, pubmed-meshheading:21256200-HeLa Cells, pubmed-meshheading:21256200-Humans, pubmed-meshheading:21256200-Microscopy, Fluorescence, pubmed-meshheading:21256200-Phenols, pubmed-meshheading:21256200-Principal Component Analysis, pubmed-meshheading:21256200-Promoter Regions, Genetic, pubmed-meshheading:21256200-Raloxifene, pubmed-meshheading:21256200-Reproducibility of Results, pubmed-meshheading:21256200-Tamoxifen
pubmed:year
2011
pubmed:articleTitle
High content imaging-based assay to classify estrogen receptor-? ligands based on defined mechanistic outcomes.
pubmed:affiliation
Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX 77030, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural