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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2011-2-8
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pubmed:abstractText |
The ability to direct functional proteins to specific DNA sequences is a long-sought goal in the study and engineering of biological processes. Transcription activator-like effectors (TALEs) from Xanthomonas sp. are site-specific DNA-binding proteins that can be readily designed to target new sequences. Because TALEs contain a large number of repeat domains, it can be difficult to synthesize new variants. Here we describe a method that overcomes this problem. We leverage codon degeneracy and type IIs restriction enzymes to generate orthogonal ligation linkers between individual repeat monomers, thus allowing full-length, customized, repeat domains to be constructed by hierarchical ligation. We synthesized 17 TALEs that are customized to recognize specific DNA-binding sites, and demonstrate that they can specifically modulate transcription of endogenous genes (SOX2 and KLF4) in human cells.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/21248753-12592412,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21248753-16971456,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21248753-17962564,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21248753-17962565,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21248753-18985154,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21248753-19282480,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/21248753-19409607,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/21248753-19448036,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21248753-19473322,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21248753-19584299,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21248753-19798082,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21248753-19816951,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21248753-19933106,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21248753-19933107,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21248753-19952142,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21248753-20007601,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21248753-20010598,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21248753-20360772,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21248753-20570209,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21248753-20660643,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21248753-21178484,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21248753-21301438,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21248753-21473016,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21248753-21822242,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21248753-9843940
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
1546-1696
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
29
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
149-53
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pubmed:dateRevised |
2011-9-20
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pubmed:meshHeading |
pubmed-meshheading:21248753-Amino Acid Sequence,
pubmed-meshheading:21248753-Bacterial Proteins,
pubmed-meshheading:21248753-Base Sequence,
pubmed-meshheading:21248753-Binding Sites,
pubmed-meshheading:21248753-Cell Line,
pubmed-meshheading:21248753-DNA,
pubmed-meshheading:21248753-Genetic Engineering,
pubmed-meshheading:21248753-High-Throughput Nucleotide Sequencing,
pubmed-meshheading:21248753-Humans,
pubmed-meshheading:21248753-Kruppel-Like Transcription Factors,
pubmed-meshheading:21248753-Microscopy, Fluorescence,
pubmed-meshheading:21248753-Molecular Sequence Data,
pubmed-meshheading:21248753-Promoter Regions, Genetic,
pubmed-meshheading:21248753-SOXB1 Transcription Factors,
pubmed-meshheading:21248753-Transcription, Genetic,
pubmed-meshheading:21248753-Transcription Factors,
pubmed-meshheading:21248753-Xanthomonas
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pubmed:year |
2011
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pubmed:articleTitle |
Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription.
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pubmed:affiliation |
Harvard University, Cambridge, Massachusetts, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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