Source:http://linkedlifedata.com/resource/pubmed/id/21248261
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
4
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pubmed:dateCreated |
2011-2-3
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pubmed:abstractText |
Capsular polysaccharides of encapsulated bacteria are weakly immunogenic T cell-independent type 2 (TI-2) Ags. Recent findings suggest that BAFF system molecules have a critical role in the development of Ab responses against TI-2 Ags. In this study, we investigated the effect of bacterial polysaccharides on B cell responses to BAFF and a proliferation-inducing ligand (APRIL). We determined that B cells exposed to meningococcal type C polysaccharide (MCPS) or group B Streptococcus serotype V (GBS-V) were unresponsive to BAFF- and APRIL-induced Ig secretion. Moreover, MCPS and GBS-V strongly downregulated transmembrane activator and calcium-modulator and cyclophilin ligand interactor, the BAFF and APRIL receptor that is responsible for Ab development against TI-2 Ags. Interestingly, (4-hydroxy-3-nitrophenyl)acetyl-Ficoll (NP-Ficoll), a prototype TI-2 Ag, did not manifest a suppressive effect on B cells. Paradoxically, whereas GBS-V and MCPS inhibited IFN-?-induced BAFF production from dendritic cells, NP-Ficoll strongly increased BAFF secretion. TLR 9 agonist CpG deoxyoligonucleotide (ODN) was able to reverse the MCPS-mediated transmembrane activator and calcium-modulator and cyclophilin ligand interactor suppression but could not rescue the Ig secretion in BAFF- or APRIL-stimulated B cells. In support of these in vitro observations, it was observed that CpG ODN could help augment the Ab response against NP in mice immunized with a CpG ODN-containing NP-Ficoll vaccine but exhibited only marginal adjuvant activity for MCPS vaccine. Collectively, these results suggest a mechanism for the weak immunogenicity of bacterial polysaccharides and explain the previously observed differences between bacterial polysaccharide and NP-Ficoll immunogenicity.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/B-Cell Activating Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Capsules,
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Vaccines,
http://linkedlifedata.com/resource/pubmed/chemical/Growth Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Immunosuppressive Agents,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B,
http://linkedlifedata.com/resource/pubmed/chemical/Polysaccharides, Bacterial,
http://linkedlifedata.com/resource/pubmed/chemical/Tnfsf13b protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/capsular polysaccharide...
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
1550-6606
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pubmed:author | |
pubmed:issnType |
Electronic
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pubmed:day |
15
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pubmed:volume |
186
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2430-43
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pubmed:meshHeading |
pubmed-meshheading:21248261-Animals,
pubmed-meshheading:21248261-B-Cell Activating Factor,
pubmed-meshheading:21248261-B-Lymphocytes,
pubmed-meshheading:21248261-Bacterial Capsules,
pubmed-meshheading:21248261-Bacterial Vaccines,
pubmed-meshheading:21248261-Cells, Cultured,
pubmed-meshheading:21248261-Dose-Response Relationship, Immunologic,
pubmed-meshheading:21248261-Growth Inhibitors,
pubmed-meshheading:21248261-Immunosuppressive Agents,
pubmed-meshheading:21248261-Mice,
pubmed-meshheading:21248261-Mice, Inbred BALB C,
pubmed-meshheading:21248261-NF-kappa B,
pubmed-meshheading:21248261-Neisseria meningitidis, Serogroup C,
pubmed-meshheading:21248261-Polysaccharides, Bacterial,
pubmed-meshheading:21248261-Signal Transduction
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pubmed:year |
2011
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pubmed:articleTitle |
Suppressive effect of bacterial polysaccharides on BAFF system is responsible for their poor immunogenicity.
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pubmed:affiliation |
Laboratory of Bacterial Polysaccharides, Division of Bacterial Parasitic and Allergenic Products, Center for Biologics Evaluation and Research, US Food and Drug Administration, Bethesda, MD 20852, USA.
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pubmed:publicationType |
Journal Article,
Comparative Study
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