| pubmed-article:21247629 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:21247629 | lifeskim:mentions | umls-concept:C0040300 | lld:lifeskim |
| pubmed-article:21247629 | lifeskim:mentions | umls-concept:C0010453 | lld:lifeskim |
| pubmed-article:21247629 | lifeskim:mentions | umls-concept:C1882071 | lld:lifeskim |
| pubmed-article:21247629 | lifeskim:mentions | umls-concept:C0018120 | lld:lifeskim |
| pubmed-article:21247629 | lifeskim:mentions | umls-concept:C1254042 | lld:lifeskim |
| pubmed-article:21247629 | lifeskim:mentions | umls-concept:C0063083 | lld:lifeskim |
| pubmed-article:21247629 | lifeskim:mentions | umls-concept:C1707689 | lld:lifeskim |
| pubmed-article:21247629 | lifeskim:mentions | umls-concept:C1707511 | lld:lifeskim |
| pubmed-article:21247629 | pubmed:issue | 10 | lld:pubmed |
| pubmed-article:21247629 | pubmed:dateCreated | 2011-2-1 | lld:pubmed |
| pubmed-article:21247629 | pubmed:abstractText | Synthetic hydrogels with tunable properties are appealing for regenerative medicine. A critical limitation in hydrogel design at low solids concentration is the formation of defects, which increase gelation times and swelling, and reduce elasticity. Here, we report that trifunctional cross-linking peptides applied to 4-arm poly-(ethylene glycol) (PEG) hydrogels decreased swelling and gelation time relative to bi-functional crosslinkers. In contrast to bi-functional peptides, the third cross-linking site on the peptide created a branch point if an intramolecular cross-link formed, which prevented non-functional "dangling-ends" in the hydrogel network and enhanced the number of elastically active cross-links. The improved network formation enabled mouse ovarian follicle encapsulation and maturation in vitro. Hydrogels with bi-functional crosslinkers resulted in cellular dehydration, likely due to osmosis during the prolonged gelation. For trifunctional crosslinkers, the hydrogels supported a 17-fold volumetric expansion of the tissue during culture, with expansion dependent on the ability of the follicle to rearrange its microenvironment, which is controlled through the sensitivity of the cross-linking peptide to the proteolytic activity of plasmin. The improved network design enabled ovarian follicle culture in a completely synthetic system, and can advance fertility preservation technology for women facing premature infertility from anticancer therapies. | lld:pubmed |
| pubmed-article:21247629 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21247629 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21247629 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21247629 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21247629 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21247629 | pubmed:language | eng | lld:pubmed |
| pubmed-article:21247629 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21247629 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:21247629 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21247629 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:21247629 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:21247629 | pubmed:issn | 1878-5905 | lld:pubmed |
| pubmed-article:21247629 | pubmed:author | pubmed-author:SheaLonnie... | lld:pubmed |
| pubmed-article:21247629 | pubmed:author | pubmed-author:WoodruffTeres... | lld:pubmed |
| pubmed-article:21247629 | pubmed:author | pubmed-author:LANVV | lld:pubmed |
| pubmed-article:21247629 | pubmed:author | pubmed-author:ShikanovAriel... | lld:pubmed |
| pubmed-article:21247629 | pubmed:author | pubmed-author:SmithRachel... | lld:pubmed |
| pubmed-article:21247629 | pubmed:copyrightInfo | Copyright © 2010 Elsevier Ltd. All rights reserved. | lld:pubmed |
| pubmed-article:21247629 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:21247629 | pubmed:volume | 32 | lld:pubmed |
| pubmed-article:21247629 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:21247629 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:21247629 | pubmed:pagination | 2524-31 | lld:pubmed |
| pubmed-article:21247629 | pubmed:dateRevised | 2011-6-14 | lld:pubmed |
| pubmed-article:21247629 | pubmed:meshHeading | pubmed-meshheading:21247629... | lld:pubmed |
| pubmed-article:21247629 | pubmed:meshHeading | pubmed-meshheading:21247629... | lld:pubmed |
| pubmed-article:21247629 | pubmed:meshHeading | pubmed-meshheading:21247629... | lld:pubmed |
| pubmed-article:21247629 | pubmed:meshHeading | pubmed-meshheading:21247629... | lld:pubmed |
| pubmed-article:21247629 | pubmed:meshHeading | pubmed-meshheading:21247629... | lld:pubmed |
| pubmed-article:21247629 | pubmed:meshHeading | pubmed-meshheading:21247629... | lld:pubmed |
| pubmed-article:21247629 | pubmed:meshHeading | pubmed-meshheading:21247629... | lld:pubmed |
| pubmed-article:21247629 | pubmed:meshHeading | pubmed-meshheading:21247629... | lld:pubmed |
| pubmed-article:21247629 | pubmed:meshHeading | pubmed-meshheading:21247629... | lld:pubmed |
| pubmed-article:21247629 | pubmed:meshHeading | pubmed-meshheading:21247629... | lld:pubmed |
| pubmed-article:21247629 | pubmed:year | 2011 | lld:pubmed |
| pubmed-article:21247629 | pubmed:articleTitle | Hydrogel network design using multifunctional macromers to coordinate tissue maturation in ovarian follicle culture. | lld:pubmed |
| pubmed-article:21247629 | pubmed:affiliation | Department of Chemical and Biological Engineering, Northwestern University, 2170 Campus Dr, 3619 Silverman Hall, Evanston, IL 60208, USA. | lld:pubmed |
| pubmed-article:21247629 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:21247629 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
