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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2011-2-4
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pubmed:abstractText |
Concerns over the health effects of nanomaterials in the environment have created a need for microscopy methods capable of examining the biological interactions of nanoparticles (NP). Unfortunately, NP are beyond the diffraction limit of resolution for conventional light microscopy (~200 nm). Fluorescence and electron microscopy techniques commonly used to examine NP interactions with biological substrates have drawbacks that limit their usefulness in toxicological investigation of NP. EM is labor intensive and slow, while fluorescence carries the risk of photobleaching the sample and has size resolution limits. In addition, many relevant particles lack intrinsic fluorescence and therefore can not be detected in this manner. To surmount these limitations, we evaluated the potential of a novel combination of darkfield and confocal laser scanning microscopy (DF-CLSM) for the efficient 3D detection of NP in human lung cells. The DF-CLSM approach utilizes the contrast enhancements of darkfield microscopy to detect objects below the diffraction limit of 200 nm based on their light scattering properties and interfaces it with the power of confocal microscopy to resolve objects in the z-plane.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/21247485-10813449,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21247485-15204759,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21247485-16002369,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/21247485-17454253,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21247485-17511644,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21247485-17872897,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21247485-17894871,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21247485-18228320,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/21247485-19238274,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/21247485-19525947,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21247485-19914270,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21247485-20205860,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21247485-20217596,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21247485-20564539,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21247485-2450641
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
1743-8977
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
8
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2
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pubmed:dateRevised |
2011-7-25
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pubmed:meshHeading |
pubmed-meshheading:21247485-Animals,
pubmed-meshheading:21247485-Cell Line,
pubmed-meshheading:21247485-Endocytosis,
pubmed-meshheading:21247485-Fluorescent Dyes,
pubmed-meshheading:21247485-Humans,
pubmed-meshheading:21247485-Lung,
pubmed-meshheading:21247485-Microscopy, Confocal,
pubmed-meshheading:21247485-Nanoparticles,
pubmed-meshheading:21247485-Particle Size,
pubmed-meshheading:21247485-Polystyrenes,
pubmed-meshheading:21247485-Titanium
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pubmed:year |
2011
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pubmed:articleTitle |
Darkfield-confocal microscopy detection of nanoscale particle internalization by human lung cells.
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pubmed:affiliation |
Environmental Public Health Division, National Health and Environmental Effects Research Laboratory, U.S. Environmental Protection Agency, Chapel Hill, NC, USA.
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pubmed:publicationType |
Journal Article
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