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pubmed:abstractText |
The protein phosphatase-2A (PP-2A), one of the major phosphatases in eukaryotes, is a heterotrimer, consisting of a scaffold A subunit, a catalytic C subunit and a regulatory B subunit. Previous studies have shown that besides regulating specific PP-2A activity, various B subunits encoded by more than 16 different genes, may have other functions. To explore the possible roles of the regulatory subunits of PP-2A in vertebrate development, we have cloned the PR55/B family regulatory subunits: ? and ?, analyzed their tissue specific and developmental expression patterns in Goldfish ( Carassius auratus). Our results revealed that the full-length cDNA for PR55/B? consists of 1940 bp with an open reading frame of 1332 nucleotides coding for a deduced protein of 443 amino acids. The full length PR55/B? cDNA is 2163 bp containing an open reading frame of 1347 nucleotides encoding a deduced protein of 448 amino acids. The two isoforms of PR55/B display high levels of sequence identity with their counterparts in other species. The PR55/B? mRNA and protein are detected in brain and heart. In contrast, the PR55/B? is expressed in all 9 tissues examined at both mRNA and protein levels. During development of goldfish, the mRNAs for PR55/B? and PR55/B? show distinct patterns. At the protein level, PR55/B? is expressed at all developmental stages examined, suggesting its important role in regulating goldfish development. Expression of the PR55/B? anti-sense RNA leads to significant downregulation of PR55/B? proteins and caused severe abnormality in goldfish trunk and eye development. Together, our results suggested that PR55/B? plays an important role in governing normal trunk and eye formation during goldfish development.
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pubmed:affiliation |
Key Laboratory of Protein Chemistry and Developmental Biology of Educational Ministry of China, College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, China.
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