Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2011-2-2
pubmed:abstractText
Shear stress, especially low shear stress (LowSS), plays an important role in vascular remodeling during atherosclerosis. Endothelial cells (ECs), which are directly exposed to shear stress, convert mechanical stimuli into intracellular signals and interact with the underlying vascular smooth muscle cells (VSMCs). The interactions between ECs and VSMCs modulate the LowSS-induced vascular remodeling. With the use of proteomic analysis, the protein profiles of rat aorta cultured under LowSS (5 dyn/cm(2)) and normal shear stress (15 dyn/cm(2)) were compared. By using Ingenuity Pathway Analysis to identify protein-protein association, a network was disclosed that involves two secretary molecules, PDGF-BB and TGF-?1, and three other linked proteins, lamin A, lysyl oxidase, and ERK 1/2. The roles of this network in cellular communication, migration, and proliferation were further studied in vitro by a cocultured parallel-plate flow chamber system. LowSS up-regulated migration and proliferation of ECs and VSMCs, increased productions of PDGF-BB and TGF-?1, enhanced expressions of lysyl oxidase and phospho-ERK1/2, and decreased Lamin A in ECs and VSMCs. These changes induced by LowSS were confirmed by using PDGF-BB recombinant protein, siRNA, and neutralizing antibody. TGF-?1 had similar influences on ECs as PDGF-BB, but not on VSMCs. Our results suggest that ECs convert the LowSS stimuli into up-regulations of PDGF-BB and TGF-?1, but these two factors play different roles in LowSS-induced vascular remodeling. PDGF-BB is involved in the paracrine control of VSMCs by ECs, whereas TGF-?1 participates in the feedback control from VSMCs to ECs.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/21245329-12032360, http://linkedlifedata.com/resource/pubmed/commentcorrection/21245329-12231551, http://linkedlifedata.com/resource/pubmed/commentcorrection/21245329-12595340, http://linkedlifedata.com/resource/pubmed/commentcorrection/21245329-12865076, http://linkedlifedata.com/resource/pubmed/commentcorrection/21245329-12898522, http://linkedlifedata.com/resource/pubmed/commentcorrection/21245329-14563712, http://linkedlifedata.com/resource/pubmed/commentcorrection/21245329-14996565, http://linkedlifedata.com/resource/pubmed/commentcorrection/21245329-15174055, http://linkedlifedata.com/resource/pubmed/commentcorrection/21245329-15568038, http://linkedlifedata.com/resource/pubmed/commentcorrection/21245329-16084198, http://linkedlifedata.com/resource/pubmed/commentcorrection/21245329-16163360, http://linkedlifedata.com/resource/pubmed/commentcorrection/21245329-16389518, http://linkedlifedata.com/resource/pubmed/commentcorrection/21245329-16477012, http://linkedlifedata.com/resource/pubmed/commentcorrection/21245329-16482415, http://linkedlifedata.com/resource/pubmed/commentcorrection/21245329-16840173, http://linkedlifedata.com/resource/pubmed/commentcorrection/21245329-17030791, http://linkedlifedata.com/resource/pubmed/commentcorrection/21245329-17251970, http://linkedlifedata.com/resource/pubmed/commentcorrection/21245329-17371946, http://linkedlifedata.com/resource/pubmed/commentcorrection/21245329-17631533, http://linkedlifedata.com/resource/pubmed/commentcorrection/21245329-18469024, http://linkedlifedata.com/resource/pubmed/commentcorrection/21245329-18535268, http://linkedlifedata.com/resource/pubmed/commentcorrection/21245329-18540023, http://linkedlifedata.com/resource/pubmed/commentcorrection/21245329-18583708, http://linkedlifedata.com/resource/pubmed/commentcorrection/21245329-18583710, http://linkedlifedata.com/resource/pubmed/commentcorrection/21245329-18596264, http://linkedlifedata.com/resource/pubmed/commentcorrection/21245329-19038849, http://linkedlifedata.com/resource/pubmed/commentcorrection/21245329-19089318, http://linkedlifedata.com/resource/pubmed/commentcorrection/21245329-19288585, http://linkedlifedata.com/resource/pubmed/commentcorrection/21245329-19390054, http://linkedlifedata.com/resource/pubmed/commentcorrection/21245329-20048148, http://linkedlifedata.com/resource/pubmed/commentcorrection/21245329-20069369
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1091-6490
pubmed:author
pubmed:issnType
Electronic
pubmed:day
1
pubmed:volume
108
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1908-13
pubmed:dateRevised
2011-8-3
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
PDGF-BB and TGF-{beta}1 on cross-talk between endothelial and smooth muscle cells in vascular remodeling induced by low shear stress.
pubmed:affiliation
Institute of Mechanobiology and Medical Engineering, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't