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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2011-5-20
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pubmed:abstractText |
The membrane protein ETHYLENE INSENSITIVE2 (EIN2), which is supposed to act between the soluble serine/threonine kinase CTR1 and the EIN3/EIL family of transcription factors, is a central and most critical element of the ethylene signaling pathway in Arabidopsis. In a recent study, we have identified that EIN2 interacts tightly with all members of the Arabidopsis ethylene receptor family - proteins that mark the starting point of the signaling pathway. Our studies show consistently that the kinase domain of the receptors is essential for the formation of the EIN2-receptor complex. Furthermore, mutational analysis demonstrates that phosphorylation is a key mechanism in controlling the interaction of EIN2 and the ethylene receptors. Interaction studies in the presence of the ethylene agonist cyanide revealed a causal link between hormone binding and complex formation. In the presence of the plant hormone agonist the auto-kinase activity of the receptors is inhibited and the non-phosphorylated kinase domain of the receptors binds tightly to the carboxyl-terminal domain of EIN2. In the absence of cyanide inhibition of the auto-kinase activity is relieved and complex formation with the phosphorylated kinase domain of the receptors is reduced. Our data suggest a novel model on the integration of EIN2 in the ethylene signaling pathway.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/21242723-10381874,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21242723-11019801,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21242723-11031228,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21242723-11258480,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21242723-19196655,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21242723-19769567,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21242723-19825547,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21242723-20591837,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21242723-8431946,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21242723-8681377,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21242723-9636235,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21242723-9928480
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
1559-2324
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
6
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
164-6
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pubmed:meshHeading |
pubmed-meshheading:21242723-Arabidopsis,
pubmed-meshheading:21242723-Arabidopsis Proteins,
pubmed-meshheading:21242723-Ethylenes,
pubmed-meshheading:21242723-Models, Biological,
pubmed-meshheading:21242723-Protein Binding,
pubmed-meshheading:21242723-Protein Structure, Tertiary,
pubmed-meshheading:21242723-Receptors, Cell Surface,
pubmed-meshheading:21242723-Signal Transduction
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pubmed:year |
2011
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pubmed:articleTitle |
New paradigm in ethylene signaling: EIN2, the central regulator of the signaling pathway, interacts directly with the upstream receptors.
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pubmed:affiliation |
Heinrich-Heine Universität Düsseldorf, Biochemische Pflanzenphysiologie, Düsseldorf, Germany.
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pubmed:publicationType |
Journal Article
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