Linked Life Data

2124205

Source:http://linkedlifedata.com/resource/pubmed/id/2124205

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1991-1-29
pubmed:abstractText
To characterize the poorly understood mechanisms of intracellular transport of bile acids, fluorescein isothiocyanate-glycocholate was synthesized and its ring-OH-linked structure established by fast atom bombardment, mass spectroscopy and 13C nuclear magnetic resonance. Biliary secretion of fluorescein isothiocyanate-glycocholate and [14C]-labeled glycocholate in rats was similar, in contrast to the biliary secretion of sodium fluorescein and methylamine-conjugated fluorescein isothiocyanate, which are non-bile acid organic anions. After incubation of cultured hepatocyte doublets with fluorescein isothiocyanate-glycocholate, serial quantitative image analysis of fluorescence was performed in the cellular cytoplasm, perinuclear zone and bile canaliculus. Uptake of fluorescein isothiocyanate-glycocholate into the cytoplasm was inhibited by removal of sodium from the medium and by addition of glycocholate or taurocholate. After preincubation with colchicine, but not lumicolchicine, the proportion of perinuclear to cytoplasmic fluorescein isothiocyanate-glycocholate increased during incubation with fluorescein isothiocyanate-glycocholate. Neither fluorescein isothiocyanate-glycocholate uptake nor canalicular secretion was affected. By confocal microscopy the perinuclear zone containing fluorescein isothiocyanate-glycocholate was identified as the Golgi apparatus by fluorescent colocalization with C6-NBD-ceramide, which specifically identifies the Golgi apparatus. We conclude that colchicine inhibits fluorescein isothiocyanate-glycocholate transfer from the Golgi apparatus to the bile canaliculus. These results suggest that intracellular bile acid transport involves microtubule-dependent vesicular movement from the Golgi apparatus to the bile canaliculus. The role of this process in normal physiology is uncertain.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0270-9139
pubmed:author
pubmed:issnType
Print
pubmed:volume
12
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1358-64
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
Serial quantitative image analysis and confocal microscopy of hepatic uptake, intracellular distribution and biliary secretion of a fluorescent bile acid analog in rat hepatocyte doublets.
pubmed:affiliation
Department of Physiology, Tufts University School of Medicine, Boston, Massachusetts 02111.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.