Linked Life Data

21238598

Source:http://linkedlifedata.com/resource/pubmed/id/21238598

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2011-2-23
pubmed:abstractText
Peroxisome proliferator-activated receptor-? (PPAR?) and CCAAT/enhancer binding protein-? (C/EBP?) are the master regulators of adipogenesis. The regulatory mechanism of PPAR? and C/EBP? gene expression is clear in mammals, however, little is known in chicken. The aim of the present study was to characterize chicken PPAR? promoter and investigate whether PPAR? could be regulated by C/EBP? in chickens. A 2-kb nucleotide sequence upstream of the start codon of chicken PPAR? gene was cloned and characterized by using bioinformatics and experimental approaches. This 2-kb promoter region exhibited strong promoter activity in DF1 cells. The reporter gene assay showed that the chicken C/EBP? could activate PPAR? gene promoter. Further study by electrophoretic mobility shift assay and mutational analysis revealed that the chicken C/EBP? could directly bind to and regulate the PPAR? gene promoter. Our results demonstrate that PPAR? can be directly regulated by C/EBP? in chickens.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1879-1107
pubmed:author
pubmed:copyrightInfo
Copyright © 2011 Elsevier Inc. All rights reserved.
pubmed:issnType
Electronic
pubmed:volume
158
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
297-303
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
Functional analysis of the chicken PPAR? gene 5'-flanking region and C/EBP?-mediated gene regulation.
pubmed:affiliation
College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, PR China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't