Linked Life Data

21235538

Source:http://linkedlifedata.com/resource/pubmed/id/21235538

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2011-3-11
pubmed:abstractText
Lymphopenia is a common clinical manifestation in patients with systemic lupus erythematosus (SLE). However, its physiopathogenic role and the contribution of different T cell subsets in this setting have not been addressed fully. The aim of this study was to characterize T cell subsets quantitatively and functionally and their association with lymphopenia and azathioprine treatment in SLE. We included 84 SLE patients and 84 healthy controls and selected 20 patients for a 6-month longitudinal analysis. Peripheral blood mononuclear cells were isolated, and T cell subsets were analysed by flow cytometry. Functional analyses included autologous and allogeneic co-cultures of T cells. Our data show persistently lower absolute numbers of CD4(+) CD25(high) T cells [regulatory T cells (T(regs) )] (1·9 versus 5·2, P?<?0·01) and CD4(+) CD69(+) T cells (3·2 versus 9·3, P?=?0·02) and higher activity scores (4·1 versus 1·5, P?=?0·01) in SLE patients with lymphopenia compared with those without lymphopenia. Lymphopenia increased the risk for decreased numbers of CD4(+) CD25(high) cells (relative risk 1·80, 95% confidence interval 1·10-2·93; P?=?0·003). In addition, azathioprine-associated lymphopenia was characterized by decreased absolute numbers of CD4(+) CD69(+) and CD4(+) interleukin (IL)-17(+) cells compared to disease activity-associated lymphopenia. Functional assays revealed that SLE effector T cells were highly proliferative and resistant to suppression by autologous T(regs) . In summary, lymphopenia was associated with deficient numbers of CD4(+) CD25(high) and CD4(+) CD69(+) cells and resistance of effector T cells to suppression by T(regs) , which could contribute to the altered immune responses characteristic of SLE. Furthermore, azathioprine treatment was associated with decreased numbers of CD4(+) CD69(+) and CD4(+) IL-17(+) cells and diminished T(reg) suppressive activity.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1365-2249
pubmed:author
pubmed:copyrightInfo
© 2011 The Authors. Clinical and Experimental Immunology © 2011 British Society for Immunology.
pubmed:issnType
Electronic
pubmed:volume
164
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
17-25
pubmed:meshHeading
pubmed-meshheading:21235538-Adult, pubmed-meshheading:21235538-Antigens, CD, pubmed-meshheading:21235538-Antigens, Differentiation, T-Lymphocyte, pubmed-meshheading:21235538-Azathioprine, pubmed-meshheading:21235538-CD4-Positive T-Lymphocytes, pubmed-meshheading:21235538-Cells, Cultured, pubmed-meshheading:21235538-Female, pubmed-meshheading:21235538-Flow Cytometry, pubmed-meshheading:21235538-Follow-Up Studies, pubmed-meshheading:21235538-Humans, pubmed-meshheading:21235538-Immunosuppressive Agents, pubmed-meshheading:21235538-Interleukin-2 Receptor alpha Subunit, pubmed-meshheading:21235538-Lectins, C-Type, pubmed-meshheading:21235538-Lupus Erythematosus, Systemic, pubmed-meshheading:21235538-Lymphocyte Count, pubmed-meshheading:21235538-Lymphopenia, pubmed-meshheading:21235538-Male, pubmed-meshheading:21235538-T-Lymphocyte Subsets, pubmed-meshheading:21235538-T-Lymphocytes, Regulatory, pubmed-meshheading:21235538-Time Factors, pubmed-meshheading:21235538-Young Adult
pubmed:year
2011
pubmed:articleTitle
Quantitative and functional profiles of CD4+ lymphocyte subsets in systemic lupus erythematosus patients with lymphopenia.
pubmed:affiliation
Department of Immunology and Rheumatology, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Mexico City, Mexico.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't