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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
10
|
| pubmed:dateCreated |
1991-1-16
|
| pubmed:abstractText |
Stimulation of T lymphocytes through their antigen receptor leads to the appearance of several transcription factors, including NF-AT and NF-kappa B, which are involved in regulating genes required for immunologic activation. To investigate the activity of a single transcription factor in individual viable cells, we have applied an assay that uses the fluorescence-activated cell sorter to quantitate beta-galactosidase (beta-gal). We have analyzed the distribution of NF-AT transcriptional activity among T cells undergoing activation by using a construct in which three tandem copies of the NF-AT-binding site directs transcription of the lacZ gene. Unexpectedly, stimulation of cloned stably transfected Jurkat T cells leads to a bimodal pattern of beta-gal expression in which some cells express no beta-gal and others express high levels. This expression pattern cannot be accounted for by cell-cycle position or heritable variation. Further results, in which beta-gal activity is correlated with NF-AT-binding activity, indicate that the concentration of NF-AT must exceed a critical threshold before transcription initiates. This threshold likely reflects the NF-AT concentration-dependent assembly of transcription complexes at the promoter. Similar constructs controlled by NF-kappa B or the entire interleukin-2 enhancer show bimodal expression patterns during induction, suggesting that thresholds set by the concentration of transcription factors may be a common property of inducible genes.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cinnamates,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Hygromycin B,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell,
http://linkedlifedata.com/resource/pubmed/chemical/Ribonucleases,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/beta-Galactosidase,
http://linkedlifedata.com/resource/pubmed/chemical/hygromycin A
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
0890-9369
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
4
|
| pubmed:geneSymbol |
lacZ
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1823-34
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:2123468-Cell Cycle,
pubmed-meshheading:2123468-Cells, Cultured,
pubmed-meshheading:2123468-Cinnamates,
pubmed-meshheading:2123468-DNA-Binding Proteins,
pubmed-meshheading:2123468-Drug Resistance,
pubmed-meshheading:2123468-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:2123468-Gene Expression Regulation,
pubmed-meshheading:2123468-Humans,
pubmed-meshheading:2123468-Hygromycin B,
pubmed-meshheading:2123468-Plasmids,
pubmed-meshheading:2123468-Receptors, Antigen, T-Cell,
pubmed-meshheading:2123468-Ribonucleases,
pubmed-meshheading:2123468-Signal Transduction,
pubmed-meshheading:2123468-T-Lymphocytes,
pubmed-meshheading:2123468-Transcription, Genetic,
pubmed-meshheading:2123468-Transcription Factors,
pubmed-meshheading:2123468-Transfection,
pubmed-meshheading:2123468-beta-Galactosidase
|
| pubmed:year |
1990
|
| pubmed:articleTitle |
Single cell assay of a transcription factor reveals a threshold in transcription activated by signals emanating from the T-cell antigen receptor.
|
| pubmed:affiliation |
Department of Genetics, Stanford University, California 94305.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|